Könönen E, Jousimies-Somer H, Asikainen S
University of Helsinki, Finland.
Oral Microbiol Immunol. 1992 Oct;7(5):273-6. doi: 10.1111/j.1399-302x.1992.tb00587.x.
Various gram-negative anaerobic bacterial species can be detected in the oral flora of edentulous infants. However, knowledge of the initial infection source is still scarce. Thirty Caucasian mothers (mean age 30 years) and their edentulous infants (mean age 3 months) were examined for the possible similarity of the oral gram-negative anaerobic flora. Paraffin-stimulated saliva was collected from the mothers. A pooled swab sample from mucosal surfaces and unstimulated saliva were collected from the infants. The samples were inoculated on nonselective and selective media and cultured aerobically and anaerobically. All of the 30 mothers harbored Fusobacterium nucleatum and 29 mothers Prevotella melaninogenica in their saliva. The salivary levels of P. melaninogenica, F. nucleatum, nonpigmented Prevotella spp., Prevotella intermedia and Prevotella loescheii exceeded 10(4) CFU/ml in about half of the 30 mothers. At this maternal salivary level, the infants' colonization frequency of P. melaninogenica and F. nucleatum was doubled. A positive correlation between maternal salivary concentration and infant's colonization was found for P. melaninogenica. No positive association was found with nonpigmented Prevotella spp., P. intermedia and P. loescheii. It can be speculated that maternal saliva may act as a source of some gram-negative anaerobes in the oral microflora of edentulous infants as early as before tooth eruption.
在无牙婴儿的口腔菌群中可检测到多种革兰氏阴性厌氧细菌。然而，关于初始感染源的了解仍然很少。对30名白人母亲（平均年龄30岁）及其无牙婴儿（平均年龄3个月）进行了口腔革兰氏阴性厌氧菌群相似性的检测。从母亲处收集石蜡刺激唾液。从婴儿处收集黏膜表面的混合拭子样本和非刺激唾液。将样本接种在非选择性和选择性培养基上，分别进行需氧和厌氧培养。30名母亲的唾液中均含有具核梭杆菌，29名母亲的唾液中含有产黑色素普雷沃菌。在30名母亲中，约半数母亲唾液中产黑色素普雷沃菌、具核梭杆菌、非色素普雷沃菌属、中间普雷沃菌和洛氏普雷沃菌的水平超过10(4) CFU/ml。在此母亲唾液水平下，婴儿中产黑色素普雷沃菌和具核梭杆菌的定植频率翻倍。发现母亲唾液浓度与婴儿中产黑色素普雷沃菌的定植之间存在正相关。未发现与非色素普雷沃菌属、中间普雷沃菌和洛氏普雷沃菌有正相关。可以推测，早在牙齿萌出之前，母亲唾液可能是无牙婴儿口腔微生物群中某些革兰氏阴性厌氧菌的来源。
