Oral Health Science Center, Tokyo Dental College, Mihama-ku, Chiba city, Chiba, Japan.
Gerodontology. 2012 Jun;29(2):e494-502. doi: 10.1111/j.1741-2358.2011.00506.x. Epub 2011 Sep 19.
The purpose of this study was to investigate colonisation by periodontopathic bacteria and the sites of colonisation in elderly upper and lower complete denture wearers. We also investigated the relationship between level of oral hygiene and colonisation by periodontopathic bacteria.
Forty edentulous and 37 dentate volunteers participated in this study. Samples were collected from whole saliva, and levels of Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia and Fusobacterium nucleatum were determined by PCR Invader technology. Detection of these species on oral mucosal and denture surfaces was performed by PCR. Fisher's exact test was used for the statistical analysis. Cluster analysis was employed to investigate trends in the periodontopathic bacteria flora in each sampling area.
Detection rates of periodontopathic bacteria in whole saliva were lower under edentulous conditions than under dentulous conditions, except for A. actinomycetemcomitans and F. nucleatum (p < 0.01). Detection rate of F. nucleatum was the highest in all areas. A positive correlation was observed between DNA quantification of P. gingivalis and number of Candida species in saliva. Cluster analysis of the test species identified two clusters. Tongue-coating status was associated with the detection rate of all periodontopathic bacteria investigated, and denture plaque status was associated with the detection rate of T. denticola and F. nucleatum.
Results indicate the presence of periodontopathic bacteria under edentulous conditions and that the status of oral hygiene of the mucosal or denture surfaces affects colonisation by T. denticola and F. nucleatum.
本研究旨在调查牙周致病菌在老年上下颌全口义齿佩戴者中的定植情况和定植部位。我们还研究了口腔卫生水平与牙周致病菌定植之间的关系。
本研究纳入了 40 名无牙颌和 37 名有牙颌志愿者。采集全唾液样本，采用 PCR Invader 技术检测牙龈卟啉单胞菌、中间普氏菌、伴放线放线杆菌、牙髓卟啉单胞菌、福赛坦纳菌和核梭杆菌的水平。采用 PCR 检测口腔黏膜和义齿表面这些物种的存在。Fisher 确切检验用于统计分析。聚类分析用于研究每个采样区域牙周致病菌菌群的趋势。
除伴放线放线杆菌和核梭杆菌外（p < 0.01），无牙颌条件下牙周致病菌在全唾液中的检出率低于有牙颌条件。所有区域中核梭杆菌的检出率最高。牙龈卟啉单胞菌 DNA 定量与唾液中念珠菌种类之间存在正相关。测试物种的聚类分析确定了两个聚类。舌苔状况与所有调查的牙周致病菌的检出率相关，义齿菌斑状况与牙髓卟啉单胞菌和核梭杆菌的检出率相关。
结果表明无牙颌条件下存在牙周致病菌，口腔黏膜或义齿表面的口腔卫生状况影响牙髓卟啉单胞菌和核梭杆菌的定植。
