Department of Immunochemistry Research, Evanston Hospital and Department of Microbiology, Northwestern University, Evanston, Illinois 60201.
Infect Immun. 1970 Jan;1(1):98-108. doi: 10.1128/iai.1.1.98-108.1970.
We have isolated from human erythrocyte ghosts a fraction which prevents the attachment of unheated as well as heated lipopolysaccharides of gram-negative bacteria to red cells. This material has no significant inhibitory effect either toward the Vi antigen of gram-negative bacteria or towards the group and common antigens of the gram-positive bacteria investigated. We, therefore, named this fraction "lipopolysaccharide receptor." The receptor interacts with lipopolysaccharides and not with erythrocytes, it forms complexes with and blocks those groupings of lipopolysaccharides which attach to red cells. The effect of the receptor is physical and not enzymatic. The interaction of the receptor with the lipopolysaccharides is reversible, and the receptor removes lipopolysaccharides fixed to red cells. An equilibrium of lipopolysaccharide distribution between cells and receptor is established when receptor-lipopolysaccharide complexes are incubated with red cells. The receptor is labile toward heat and deviation of the hydrogen ion concentration from neutrality; aldehydes destroy its inhibitory activity.
我们已经从人红细胞中分离出一种物质，它能阻止未经加热和加热的革兰氏阴性菌脂多糖与红细胞的附着。该物质对革兰氏阴性菌的 Vi 抗原以及我们研究的革兰氏阳性菌的群和共同抗原没有明显的抑制作用。因此，我们将该物质命名为“脂多糖受体”。受体与脂多糖相互作用，而不是与红细胞相互作用，它与附着在红细胞上的脂多糖形成复合物并阻断这些脂多糖的结合。该受体的作用是物理性的，而不是酶性的。受体与脂多糖的相互作用是可逆的，受体可以去除固定在红细胞上的脂多糖。当受体-脂多糖复合物与红细胞孵育时，在细胞和受体之间建立脂多糖的平衡分布。该受体对热不稳定，氢离子浓度偏离中性也会使其失活；醛类会破坏其抑制活性。
