Endris R G, Hess W R
Plum Island Animal Disease Center, USDA-ARS-NAA, Greenport, New York 11944-0848.
J Med Entomol. 1992 Jul;29(4):652-6. doi: 10.1093/jmedent/29.4.652.
A total of 1,600 Ornithodoros (Pavlovskyella) marcocanus larvae were fed on a pig with a viremia of 10(7.4) HAd50/ml of African swine fever virus (ASFV). Infected larvae were sampled daily for 15 d, and nymphs were sampled at least once per instar until they became adults. Initial titers of 10(4.48) HAd50 per larva declined to 10(4.04) within 2 d. Larval titers reached a maximum of 10(6.0) HAd50 per larva 10 d after the infective blood meal. Nymphs of each instar were fed on a susceptible pig and in each case transmitted ASFV by bite. Virus titers for first to fourth instars ranged from 10(4.61) to 10(3.34) HAd50 per nymph. Transstadial survival occurred in subsequent first, second, third, and fourth instars with an 89% survival rate over 250 d. Approximately 30% of adult ticks that were infected as larvae remained infected and transmitted ASFV to susceptible pigs 588 d later. In addition, ASFV was recovered from the same adult ticks 655 d after the infective blood meal.
总共1600只马尔科卡努钝缘蜱（Pavlovskyella属）幼虫以一头非洲猪瘟病毒（ASFV）病毒血症为10(7.4) HAd50/ml的猪为食。感染后的幼虫连续15天每天取样，若虫在每个龄期至少取样一次，直至发育为成虫。每只幼虫的初始滴度为10(4.48) HAd50，在2天内降至10(4.04)。感染性血餐10天后，幼虫滴度达到每只幼虫10(6.0) HAd50的最大值。每个龄期的若虫都以易感猪为食，并且在每种情况下都通过叮咬传播ASFV。第一至第四龄若虫的病毒滴度范围为每只若虫10(4.61)至10(3.34) HAd50。在随后的第一、第二、第三和第四龄期发生了跨期存活，250天内的存活率为89%。约30%在幼虫期感染的成年蜱在588天后仍保持感染状态，并将ASFV传播给易感猪。此外，在感染性血餐655天后，从同一成年蜱中分离出了ASFV。
