Bagni C, Mariottini P, Terrenato L, Amaldi F
Dipartimento di Biologia, II Università di Roma Tor Vergata, Italy.
Mol Gen Genet. 1992 Jul;234(1):60-4. doi: 10.1007/BF00272345.
Ribosomal protein synthesis is regulated by controlling the fraction of mRNA associated with polysomes. It is known that this value changes in different developmental stages during Xenopus embryogenesis or, more generally, with changing cell growth conditions. We present here an analysis of the proportion of mRNA loaded on polysomes, carried out with probes for five different ribosomal proteins on several batches of Xenopus embryos obtained from different individuals. The results obtained indicate the existence of probe-dependent and individual differences, which reflect genetic variations in the cis- and trans-acting regulatory elements responsible for translational regulation. The fraction of ribosomal protein mRNA loaded onto polysomes can be used as an index of an individual's capacity for ribosome production.
核糖体蛋白合成通过控制与多核糖体相关的mRNA比例来调节。已知在非洲爪蟾胚胎发育的不同阶段，或者更普遍地说，随着细胞生长条件的变化，这个值会发生改变。我们在此展示了对多核糖体上负载的mRNA比例的分析，该分析使用针对五种不同核糖体蛋白的探针，对从不同个体获得的几批非洲爪蟾胚胎进行检测。所获得的结果表明存在探针依赖性和个体差异，这反映了负责翻译调控的顺式和反式作用调控元件中的遗传变异。负载在多核糖体上的核糖体蛋白mRNA比例可作为个体核糖体生产能力的一个指标。
