Pierandrei-Amaldi P, Beccari E, Bozzoni I, Amaldi F
Cell. 1985 Aug;42(1):317-23. doi: 10.1016/s0092-8674(85)80127-6.
We have studied the regulation of ribosomal protein (r-protein) synthesis in Xenopus anucleolate mutants, which lack the genes for rRNA. The accumulation of mRNA for the two r-proteins analyzed parallels the controls up to stage 30. This mRNA is mobilized onto polysomes and is translated as in normal embryos, but r-proteins are unstable in the absence of rRNA to assemble with. A translational control of rp-mRNA distribution between polysomes and mRNPs is observed, but this is not due to an autogenous regulation by r-proteins. After stage 30 the amount of rp-mRNA declines specifically in the mutants because the transcripts are unstable. Considering the temporal correlation between this event and the onset of r-protein synthesis we suggest that an autogenous control operates at the level of transcript stability.
我们研究了非洲爪蟾无核仁突变体中核糖体蛋白（r蛋白）合成的调控，这些突变体缺乏rRNA基因。所分析的两种r蛋白的mRNA积累在30阶段之前与对照相似。这种mRNA被转运到多聚核糖体上并像在正常胚胎中一样被翻译，但在没有与之组装的rRNA的情况下，r蛋白不稳定。观察到rp - mRNA在多聚核糖体和mRNA - 核糖核蛋白颗粒之间分布的翻译控制，但这不是由于r蛋白的自体调控。30阶段之后，rp - mRNA的量在突变体中特异性下降，因为转录本不稳定。考虑到这一事件与r蛋白合成开始之间的时间相关性，我们认为自体控制在转录本稳定性水平上起作用。
