Łos Marcin, Czyz Agata, Sell Eugenia, Wegrzyn Alicja, Neubauer Peter, Wegrzyn Grzegorz
Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland.
J Appl Genet. 2004;45(1):111-20.
Infection of bacterial cultures by bacteriophages as well as prophage induction in the host cells are serious problems in both research and biotechnological laboratories. Generally, prevention strategies (like good laboratory/factory hygiene, sterilisation, decontamination and disinfection) are necessary to avoid bacteriophage contamination. However, it is well known that no matter how good the laboratory/factory practice and hygiene are, bacteriophage infections occur from time to time. The use of immunised or resistant bacterial strains against specific phages may be helpful, but properties of the genetically modified strains resistant to phages are often worse (from the point of view of a researcher or a biotechnological company) than those of the parental, phage-sensitive strains. In this article we review recent results that may provide a simple way to minimise deleterious effects of bacteriophage infection and prophage induction. It appears that low bacterial growth rates result in a significant inhibition of lytic development of various bacteriophages. Moreover, spontaneous prophage induction is less frequent in slowly growing bacteria.
噬菌体对细菌培养物的感染以及宿主细胞中的原噬菌体诱导，在研究实验室和生物技术实验室中都是严重问题。通常，预防策略（如良好的实验室/工厂卫生、灭菌、去污和消毒）对于避免噬菌体污染是必要的。然而，众所周知，无论实验室/工厂操作和卫生状况有多好，噬菌体感染仍会不时发生。使用针对特定噬菌体的免疫或抗性细菌菌株可能会有所帮助，但从研究人员或生物技术公司的角度来看，对噬菌体具有抗性的基因改造菌株的特性往往比亲本的噬菌体敏感菌株更差。在本文中，我们综述了近期的研究结果，这些结果可能提供一种简单的方法来最小化噬菌体感染和原噬菌体诱导的有害影响。似乎低细菌生长速率会显著抑制各种噬菌体的裂解发育。此外，在生长缓慢的细菌中，自发原噬菌体诱导的频率较低。
