Siede Reinhold, Büchler Ralph
Hessisches Dienstleistungszentrum für Landwirtschaft, Gartenbau und Naturschutz, Bieneninstitut Kirchhain.
Berl Munch Tierarztl Wochenschr. 2004 Jan-Feb;117(1-2):12-5.
We gathered dead bees of 56 Hessian bee colonies following a sudden collapse during winter 2002/03. Viral RNA was purified from ten dead bees per sample. Kashmir bee virus (KBV) was detected by use of a RT-PCR protocol. 13 samples were positive for KBV. The PCR amplicon was sequenced. A BLAST GenBank search clearly identified the Hessian amplicon as a KBV fragment. Similarities of more than 85% were found. Phylogenetic analysis revealed a close genetic relationship of the Hessian isolate to an isolate from New Zealand. The Northamerican, the Russian and Australian notations listed in GenBank did not cluster round with the Hessian isolate. This is the first documented detection of KBV in Middle Europe.
我们收集了2002/03年冬季突然崩溃的56个黑森蜂群的死蜂。从每个样本的10只死蜂中纯化病毒RNA。使用逆转录聚合酶链反应（RT-PCR）方案检测克什米尔蜜蜂病毒（KBV）。13个样本的KBV呈阳性。对PCR扩增子进行测序。通过GenBank数据库搜索明确鉴定出黑森扩增子为KBV片段。发现相似度超过85%。系统发育分析表明，黑森分离株与来自新西兰的分离株有密切的遗传关系。GenBank中列出的北美、俄罗斯和澳大利亚的毒株没有与黑森分离株聚类。这是在中欧首次有记录检测到KBV。
