Dianzani C, Calvieri S, Pierangeli A, Degener A M
Institute of Dermatological Clinic, Section of Virology, "La Sapienza" University, Rome, Italy.
New Microbiol. 2004 Jan;27(1):65-9.
The association of Human Papillomaviruses (HPV) DNA with female genital lesions has been widely documented whereas little has been reported about male genital pathologies. The aim of this work was to investigate the presence of HPV DNA and the genotype involved in male dysplastic genital lesions. All samples were analysed by polymerase chain reaction (PCR) to amplify HPV E1 and L1 genes. The PCR products were subjected to restriction fragment length polymorphism (RFLP) to determine the HPV genotype. We analysed 209 male genital biopsies from different lesions: mostly from acuminate condylomata and from Buschke-Lowenstein tumours, Bowen papulosis, leukoplakia of the glans, scrotal lymphangioma, penile horn and penile/perianal verrucous carcinoma. Our results revealed the constant presence of viral DNA in genital condylomata, mainly associated with low risk HPV; the presence of the same genotypes was also detected in some of the examined rare pathologies.
人乳头瘤病毒（HPV）DNA与女性生殖器病变的关联已有大量文献记载，而关于男性生殖器病变的报道则较少。本研究的目的是调查HPV DNA的存在情况以及涉及男性发育异常生殖器病变的基因型。所有样本均通过聚合酶链反应（PCR）进行分析，以扩增HPV E1和L1基因。PCR产物进行限制性片段长度多态性分析（RFLP）以确定HPV基因型。我们分析了209例来自不同病变的男性生殖器活检样本：大多数来自尖锐湿疣和Buschke-Lowenstein肿瘤、鲍温样丘疹病、龟头白斑、阴囊淋巴管瘤、阴茎角以及阴茎/肛周疣状癌。我们的结果显示，病毒DNA在生殖器湿疣中持续存在，主要与低风险HPV相关；在一些罕见病变中也检测到了相同的基因型。
