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[应用多对引物聚合酶链反应鉴别尖锐湿疣与假性湿疣]

[Differentiation of condylomata acuminata from pseudocondyloma by using multiple primer pairs polymerase chain reaction].

作者信息

Liu Y, Wang J, Wang X, Fang K

机构信息

PUMC Hospital, CAMS and PUMC, Beijing.

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1996 Aug;18(4):298-300.

PMID:9388980
Abstract

Condylomata acuminata were differentiated from pseudocondyloma by using multiple primer pairs polymerase chain reaction (PCR) technique. The paraffin-embedded tissues of 95 cases of condylomata acuminata and 33 cases of pseudocondyloma were detected for HPV DNA. Results show that HPV 6/11 DNA are found in 86/95 (90.5%) cases of condylomata acuminata, and no HPV DNA is detected in 33 cases of pseudocondyloma. The results suggest that pseudocondyloma is not associated with HPV. Multiple primer pairs PCR technique is a preferable method for differentiating condylomata acuminata from pseudocondyloma.

摘要

采用多重引物对聚合酶链反应(PCR)技术区分尖锐湿疣与假性湿疣。对95例尖锐湿疣和33例假性湿疣的石蜡包埋组织进行人乳头瘤病毒(HPV)DNA检测。结果显示,86/95(90.5%)例尖锐湿疣中检测到HPV 6/11 DNA,33例假性湿疣中未检测到HPV DNA。结果提示假性湿疣与HPV无关。多重引物对PCR技术是区分尖锐湿疣与假性湿疣的一种较好方法。

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