Isa Adiba, Priftakis Peter, Broliden Kristina, Gustafsson Britt
Department of Immunology, Microbiology, and Pathology, Division of Clinical Virology, Huddinge University Hospital, Karolinska Institutet, Stockholm, Sweden.
Pediatr Blood Cancer. 2004 Apr;42(4):357-60. doi: 10.1002/pbc.20001.
There has been much speculation about the cause of childhood acute lymphoblastic leukemia (ALL). It has been suggested, on the basis of findings in epidemiological studies, that ALL may be initiated by an in utero infection of the fetus. The human parvovirus B19 (B19) is etiologically related to human diseases, including erythema infectiosum and aplastic crisis, but it has not yet been considered to be involved in the development of ALL. Therefore, the aim of this study was to investigate, whether prenatal B19 infection could still be indirectly correlated with the development of childhood ALL.
Fifty-four Guthrie cards, collected at 3-5 days of age, from Swedish children who subsequently developed ALL, as well as from 50 healthy controls, were investigated by nested PCR for the presence of B19 DNA.
B19 DNA was not detected in any of the Guthrie cards from ALL patients or from healthy controls, although all tested samples had amplifiable cellular DNA as confirmed by an HLA DQ specific PCR.
B19 DNA was not found in any of the Guthrie cards from children who later developed ALL or in the healthy controls. These findings suggest that it is less likely that childhood ALL is associated with an in utero in fection with B19.
关于儿童急性淋巴细胞白血病（ALL）的病因一直存在诸多猜测。基于流行病学研究结果，有人提出ALL可能由胎儿宫内感染引发。人类细小病毒B19（B19）在病因上与包括传染性红斑和再生障碍危象在内的人类疾病相关，但尚未被认为与ALL的发生有关。因此，本研究的目的是调查产前B19感染是否仍可能与儿童ALL的发生间接相关。
通过巢式PCR检测54份出生3 - 5天时采集的Guthrie卡片，这些卡片来自后来患ALL的瑞典儿童以及50名健康对照，以检测B19 DNA的存在情况。
在ALL患者或健康对照的任何一张Guthrie卡片中均未检测到B19 DNA，尽管通过HLA DQ特异性PCR证实所有检测样本均有可扩增的细胞DNA。
在后来患ALL的儿童或健康对照的任何一张Guthrie卡片中均未发现B19 DNA。这些发现表明儿童ALL与B19宫内感染相关的可能性较小。
