Rodrigo Juan Pablo, García-Pedrero Juana María, González María Victoria, Fernández María Pilar, Suárez Carlos, Herrero Agustín
Department of Otolaryngology, Hospital Central Universitario de Asturias, Instituto Universitario de Oncología, Universidad de Oviedo, Spain.
Arch Otolaryngol Head Neck Surg. 2004 Feb;130(2):211-5. doi: 10.1001/archotol.130.2.211.
To examine the expression pattern of annexin A1 in normal and chronically inflamed nasal mucosa to investigate its possible role in nasal inflammation.
Immunohistochemical analysis.
Samples of middle turbinates from 5 healthy subjects and 5 patients with perennial rhinitis, and samples of nasal polyps from 7 patients.
Annexin A1 expression was examined with a standard immunohistochemical protocol on paraffin-embedded sections.
Annexin A1 was highly expressed by ciliated cells, where it was concentrated on the apical surface and within the cilia. Goblet cells and nondifferentiated basal epithelial cells did not stain. In the glands of the lamina propria, intense staining was found in the cytoplasm and in the nuclei of the cells in the duct epithelium, whereas acinar cells did not stain. Intense cytoplasmic staining was observed in infiltrating polymorphonuclear cells and macrophages. No differences in the pattern or the level of expression of annexin A1 were found in the epithelial cells and glands of normal and chronically inflamed (perennial rhinitis or polyps) nasal mucosa.
These results suggest that the expression of annexin A1 in respiratory epithelium of nasal mucosa is related to cell type and differentiation status of the cells and is not significantly altered by inflammatory diseases.
检测膜联蛋白A1在正常及慢性炎症鼻黏膜中的表达模式，以研究其在鼻炎症中的可能作用。
免疫组织化学分析。
5名健康受试者和5名常年性鼻炎患者的中鼻甲样本，以及7名患者的鼻息肉样本。
采用标准免疫组织化学方法检测石蜡包埋切片中膜联蛋白A1的表达。
纤毛细胞高度表达膜联蛋白A1，其集中于顶端表面和纤毛内。杯状细胞和未分化的基底上皮细胞不着色。在固有层腺体中，导管上皮细胞的细胞质和细胞核内可见强烈染色，而腺泡细胞不着色。浸润的多形核细胞和巨噬细胞可见强烈的细胞质染色。在正常和慢性炎症（常年性鼻炎或鼻息肉）鼻黏膜的上皮细胞和腺体中，膜联蛋白A1的表达模式或水平未发现差异。
这些结果表明，鼻黏膜呼吸上皮中膜联蛋白A1的表达与细胞类型和细胞分化状态有关，且不受炎症性疾病的显著影响。
