Park Joong Kon, Jung Jae Yong, Park Youn Hee
Department of Chemical Engineering, Kyungpook National University, Taegu 702-701, Republic of Korea.
Biotechnol Lett. 2003 Dec;25(24):2055-9. doi: 10.1023/b:bile.0000007065.63682.18.
The addition of 1% (v/v) ethanol to the basal medium inhibited growth of Gluconacetobacter hansenii but decreased the numbers of non-cellulose producing cells. Cellulose production increased 1.7 times to approx. 2.5 g l(-1) and showed a pattern of mixed growth-associated production. Microbial cells produced rigid pellicle-type bacterial cellulose as the shell of a large lump of bacterial cellulose like a static culture. The inoculum cultivated for 3 d maintained cellulose production by the fifth batch.
在基础培养基中添加1%(v/v)乙醇会抑制汉逊醋杆菌的生长,但会减少不产生纤维素的细胞数量。纤维素产量增加了1.7倍,达到约2.5 g l⁻¹,并呈现出混合生长相关生产的模式。微生物细胞产生了坚硬的薄膜状细菌纤维素,作为一大团细菌纤维素的外壳,类似于静态培养。培养3天的接种物在第五批培养时仍能维持纤维素的产量。