Cellulose and Paper Department, National Research Centre, Cairo, 12622, Dokki, Egypt.
Microbial Chemistry Department, National Research Centre, Cairo, 12622, Dokki, Egypt.
Microb Cell Fact. 2023 Feb 6;22(1):24. doi: 10.1186/s12934-023-02031-3.
Bacterial Cellulose (BC) is still the most renewable available biopolymer produced in fine nature from alternative microbial sources as bacteria. In the present study, newly BC producing bacteria were successfully isolated from acidic fruits. The most potent producer was isolated from strawberry and identified genetically using 16 s rRNA technique as Achromobacter S3. Different fruit peels were screened to produce BC using the cheapest culture medium. Among them, Mango peel waste (MPW) hydrolysate proved to be the significant inducible alternative medium without any extra nutrients for the maximum productivity. Improvement of the BC yield was successfully achieved via statistical optimization of the MPW culture medium, from 0.52 g/L to 1.22 g/L with 2.5-fold increased about the standard HS culture medium. Additionally, the physicochemical analysis affirmed the cellulose molecular structure as well as observed the crystallinity of nanofiber as 72 and 79% for BC produced by Achromobacter S33 on HS and MPW media, respectively. Moreover, the topographical study illustrated that the BC nanofibers had close characteristics upon fiber dimeter and length as about 10 and 200 nm, respectively.
细菌纤维素 (BC) 仍然是最可再生的生物聚合物，它是从细菌等替代微生物来源在精细自然中产生的。在本研究中，成功地从酸性水果中分离出了新的 BC 生产菌。从草莓中分离出的最强生产菌通过 16s rRNA 技术鉴定为不动杆菌 S3。使用最便宜的培养基筛选不同的果皮来生产 BC。其中，芒果皮废料 (MPW) 水解物被证明是一种没有任何额外营养物质的有效诱导替代培养基，可以实现最大生产力。通过对 MPW 培养基进行统计优化，成功提高了 BC 的产量，从 0.52g/L 提高到 1.22g/L，比标准 HS 培养基提高了 2.5 倍。此外，物理化学分析证实了纤维素的分子结构，并观察到由不动杆菌 S33 在 HS 和 MPW 培养基上分别生产的纳米纤维的结晶度为 72%和 79%。此外，形貌研究表明，BC 纳米纤维在纤维直径和长度上具有相似的特征，分别约为 10nm 和 200nm。
