Ozat Mustafa, Gungor Tayfun, Barun Sureyya, Demirogullari Billur, Sokmensuer Lale Karakoc, Gulbahar Ozlem, Gursoy Devrim, Muftuoglu Sevda
Zekai Tahir Burak Women's Health Education and Research Hospital, Ankara, Turkey.
Exp Toxicol Pathol. 2009 Sep;61(5):519-27. doi: 10.1016/j.etp.2009.06.007. Epub 2009 Jul 23.
Ovarian torsion is a surgical emergency affecting not only the ipsilateral ovary but also contralateral ovary. Although the conventional treatment is salpingo-oophorectomy, recent studies advocate detorsion. We hypothesized that iloprost, an analogue of prostacyclin with cytoprotective properties, may prevent the harmful effects of ischaemia-reperfusion injury in bilateral ovaries after unilateral ovarian torsion-detorsion in rat. In this study, 24 female Wistar-albino female rats were divided into four groups. Ovarian torsion was produced by applying vascular clamps to right ovaries. In Group I, bilateral oophorectomy was performed. In group II, bilateral oophorectomy was performed after a unilateral torsion period of 4h. In group III, bilateral ovaries were removed, following unilateral torsion-detorsion periods each lasted for 4h. Saline was injected i.p. 30 min before detorsion. In group IV, same experimental protocol, which was conducted in group III, was repeated. Iloprost was injected i.p. 30 min before detorsion instead of saline in group IV. Tissue levels of malondialdehyde (MDA) and nitric oxide (NO), which are the indicators for oxidative stress were determined and histopathological evaluation was performed in bilateral ovaries in all groups. The MDA and NO levels for ipsilateral ovaries of four groups were compared and no significant difference was found (p>0.05). The same comparison were done for the contralateral sides and no difference was seen either (p>0.05). In histological examination, iloprost produced improvement in I/R-induced alterations in ipsilateral and contralateral ovaries. In conclusion, these results showed that iloprost has beneficial effect on the histological appearances in both the ipsilateral and contralateral rat ovaries after unilateral torsion-detorsion.
卵巢扭转是一种外科急症，不仅会影响患侧卵巢，还会影响对侧卵巢。尽管传统治疗方法是输卵管卵巢切除术，但最近的研究主张进行扭转复位术。我们假设，伊洛前列素（一种具有细胞保护特性的前列环素类似物）可能会预防大鼠单侧卵巢扭转复位术后双侧卵巢缺血再灌注损伤的有害影响。在本研究中，将24只雌性Wistar白化大鼠分为四组。通过对右侧卵巢施加血管夹造成卵巢扭转。第一组进行双侧卵巢切除术。第二组在单侧扭转4小时后进行双侧卵巢切除术。第三组在单侧扭转复位各持续4小时后切除双侧卵巢。在扭转复位前30分钟腹腔注射生理盐水。第四组重复第三组的相同实验方案。在第四组中，在扭转复位前30分钟腹腔注射伊洛前列素而非生理盐水。测定丙二醛（MDA）和一氧化氮（NO）的组织水平（它们是氧化应激的指标），并对所有组的双侧卵巢进行组织病理学评估。比较四组患侧卵巢的MDA和NO水平，未发现显著差异（p>0.05）。对侧也进行了相同的比较，也未发现差异（p>0.05）。在组织学检查中，伊洛前列素改善了同侧和对侧卵巢因缺血再灌注引起的改变。总之，这些结果表明，伊洛前列素对大鼠单侧扭转复位术后同侧和对侧卵巢的组织学表现具有有益作用。
