改进猪10号染色体与人类1号、9号和10号染色体的比较图谱。
Improving the comparative map of porcine chromosome 10 with respect to human chromosomes 1, 9 and 10.
作者信息
Aldenhoven J, Chen Y, Backofen B, Moran C
机构信息
Centre for Advanced Technologies in Animal Genetics and Reproduction, Faculty of Veterinary Science, University of Sydney, Sydney, NSW, Australia.
出版信息
Cytogenet Genome Res. 2003;102(1-4):121-7. doi: 10.1159/000075736.
ZOO-FISH mapping shows human chromosomes 1, 9 and 10 share regions of homology with pig chromosome 10 (SSC10). A more refined comparative map of SSC10 has been developed to help identify positional candidate genes for QTL on SSC10 from human genome sequence. Genes from relevant chromosomal regions of the public human genome sequence were used to BLAST porcine EST databases. Primers were designed from the matching porcine ESTs to assign them to porcine chromosomes using the INRA somatic cell hybrid panel (INRA-SCHP) and the INRA-University of Minnesota Radiation Hybrid Panel (IMpRH). Twenty-eight genes from HSA1, 9 and 10 were physically mapped: fifteen to SSC10 (ACO1, ATP5C1, BMI1, CYB5R1, DCTN3, DNAJA1, EPHX1, GALT, GDI2, HSPC177, OPRS1, NUDT2, PHYH, RGS2, VIM), eleven to SSC1 (ADFP, ALDHIB1, CLTA, CMG1, HARC, PLAA, STOML2, RRP40, TESK1, VCP and VLDLR) and two to SSC4 (ALDH9A1 and TNRC4). Two anonymous markers were also physically mapped to SSC10 (SWR1849 and S0070) to better connect the physical and linkage maps. These assignments have further refined the comparative map between SSC1, 4 and 10 and HSA1, 9 and 10.
荧光原位杂交-荧光强度扫描（ZOO-FISH）图谱显示，人类染色体1、9和10与猪的第10号染色体（SSC10）存在同源区域。现已绘制出更精细的SSC10比较图谱，以帮助从人类基因组序列中识别SSC10上数量性状基因座（QTL）的位置候选基因。利用公共人类基因组序列相关染色体区域的基因对猪的表达序列标签（EST）数据库进行了BLAST分析。根据匹配的猪EST设计引物，使用法国国家农业研究院体细胞杂交板（INRA-SCHP）和法国国家农业研究院-明尼苏达大学辐射杂交板（IMpRH）将其定位到猪染色体上。对来自人类1号、9号和10号染色体的28个基因进行了物理定位：15个定位于SSC10（ACO1、ATP5C1、BMI1、CYB5R1、DCTN3,、DNAJA1、EPHX1、GALT、GDI2、HSPC177、OPRS1、NUDT2、PHYH、RGS2、VIM），11个定位于SSC1（ADFP、ALDHIB1、CLTA、CMG1、HARC、PLAA、STOML2、RRP40、TESK1、VCP和VLDLR），2个定位于SSC4（ALDH9A1和TNRC4）。还将两个匿名标记物理定位到SSC10（SWR1849和S0070），以更好地连接物理图谱和连锁图谱。这些定位进一步完善了SSC1、4和10与人类1号、9号和10号染色体之间的比较图谱。
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