Kasai F, Garcia C, Arruga M V, Ferguson-Smith M A
Molecular Cytogenetics Group, Cambridge University Centre for Veterinary Science, Cambridge, UK.
Cytogenet Genome Res. 2003;102(1-4):326-30. doi: 10.1159/000075770.
Chromosome-specific paints from macrochromosomes 1-9 and Z of the chicken were hybridised to metaphases of the red-legged partridge and revealed no inter-chromosomal rearrangements. The results from chromosome painting are similar to previous studies on the Japanese quail but different from findings in guinea fowl and several species of pheasant. The difference in centromere position in chicken and partridge chromosome 4, previously assumed to be the result of an inversion, was confirmed. However, FISH mapping of BAC clones from chicken chromosome 4 revealed that the order of loci was the same in both species, indicating the occurrence of a neocentromere during divergence.
来自鸡的1-9号常染色体和Z染色体的染色体特异性涂染探针与红腿鹧鸪的中期染色体进行杂交，结果显示没有染色体间重排。染色体涂染的结果与之前对日本鹌鹑的研究相似，但与珍珠鸡和几种雉鸡的研究结果不同。鸡和鹧鸪4号染色体着丝粒位置的差异（之前认为是倒位的结果）得到了证实。然而，对鸡4号染色体BAC克隆的荧光原位杂交定位显示，两个物种中基因座的顺序相同，这表明在物种分化过程中出现了新着丝粒。
