Cruz M L, Williamson D H
Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford, U.K.
Biochem J. 1992 Aug 1;285 ( Pt 3)(Pt 3):773-8. doi: 10.1042/bj2850773.
Meal-fed (3 h) rats had a decreased food intake, body weight and carcass fat compared with rats fed ad libitum. On refeeding a chow meal containing [1-14C]triolein, the production of 14CO2 was lower (45%) and the accumulation of carcass [14C]lipid higher (37%) in the meal-fed rats. There was higher lipoprotein lipase activity and greater accumulation of [14C]lipid in the epididymal and subcutaneous adipose-tissue depots of the meal-fed rats. In contrast, heparin-releasable lipoprotein lipase was not increased in perfused hearts of meal-fed rats on refeeding. Return of meal-fed rats to feeding ad libitum reversed these changes before the restoration of body weight or carcass fat. Evidence is presented that decreased dietary intake rather than meal pattern is an important determinant of the alterations in adipose lipid metabolism in the meal-fed rat in response to a meal.
与自由采食的大鼠相比，定时喂食（3小时）的大鼠食物摄入量、体重和胴体脂肪减少。在再次喂食含[1-14C]甘油三酯的饲料后，定时喂食的大鼠体内14CO2的生成量较低（45%），而胴体[14C]脂质的蓄积量较高（37%）。定时喂食的大鼠附睾和皮下脂肪组织库中的脂蛋白脂肪酶活性较高，[14C]脂质蓄积量也更大。相比之下，再次喂食时，定时喂食的大鼠灌注心脏中可被肝素释放的脂蛋白脂肪酶并未增加。定时喂食的大鼠恢复自由采食后，在体重或胴体脂肪恢复之前，这些变化就发生了逆转。有证据表明，饮食摄入量的减少而非进食模式是定时喂食的大鼠在进食后脂肪脂质代谢改变的重要决定因素。
