Paik H S, Yearick E S
J Nutr. 1978 Nov;108(11):1798-805. doi: 10.1093/jn/108.11.1798.
Activities of NaCl-inactivated lipoprotein lipase (LPL) and protamine-resistant hormone-sensitive lipase (HSL) in adipose tissue, accumulation of carcass fat, and serum triglycerides (TG) were determined in meal-fed (MF) and ad libitum-fed (AD) rats. At each feeding frequency, diets provided total fat as 15 or 30% of calories and polyunsaturated fatty acids (PUFA) as 2.5 or 11% of calories. The average energy intake of MF rats was 67% that of AD rats. Total weight gained by MF rats was only 60% that of the AD rats. Significantly greater activities of LPL, HSL, and LPL:HSL in adipose of MF rats suggested a greater capacity for fat accumulation which was not realized at the limited energy intake. In AD rats, the percentage of body fat was significantly correlated with LPL:HSL and with serum TG, suggesting that the relative enzyme activities and fat deposition may be influenced by the concentration of circulating TG. Mean body fat of rats receiving the 30% fat diet was significantly greater than that of rats fed 15% fat. Both serum TG and adipose LPL activity were significantly reduced when the diet contained high levels of PUFA.
在定时喂食（MF）和自由采食（AD）的大鼠中，测定了脂肪组织中氯化钠灭活的脂蛋白脂肪酶（LPL）和鱼精蛋白抗性激素敏感性脂肪酶（HSL）的活性、胴体脂肪的积累以及血清甘油三酯（TG）水平。在每种喂食频率下，日粮提供的总脂肪占热量的15%或30%，多不饱和脂肪酸（PUFA）占热量的2.5%或11%。MF大鼠的平均能量摄入量是AD大鼠的67%。MF大鼠的总体重仅为AD大鼠的60%。MF大鼠脂肪组织中LPL、HSL的活性以及LPL:HSL显著更高，这表明其脂肪积累能力更强，但在有限的能量摄入情况下并未实现。在AD大鼠中，体脂百分比与LPL:HSL以及血清TG显著相关，这表明相对酶活性和脂肪沉积可能受循环TG浓度的影响。接受30%脂肪日粮的大鼠的平均体脂显著高于喂食15%脂肪日粮的大鼠。当日粮中含有高水平的PUFA时，血清TG和脂肪组织LPL活性均显著降低。
