Kelly D J, Prendergast P J
Centre for Bioengineering, Department of Mechanical Engineering, Trinity College, Dublin, Ireland.
Med Biol Eng Comput. 2004 Jan;42(1):9-13. doi: 10.1007/BF02351005.
The structure and functionality of tissue-engineered cartilage is determined by the tissue culture conditions and mechanical conditioning during growth. The quality of tissue-engineered cartilage can be evaluated using tests such as the confined compression test. Tissue-engineered cartilage constructs usually consist of an outer layer of cartilage and an inner core of either undeveloped cartilage or degrading scaffold material. A biphasic poro-elastic finite element model was used to demonstrate how such a core influences the reaction force-time curve obtained from a confined compression test. The finite element model predicted that higher volumes of degraded scaffold in the inner core would reduce the aggregate modulus calculated from the confined compression test and raised the estimate of tissue permeability. The predicted aggregate modulus reduced from 0.135 MPa, for a homogenous construct, to 0.068 MPa, for a construct that was only 70% cartilaginous. It was found that biphasic poro-elastic finite modelling should be used in preference to a one-dimensional model that assumed homogeneity in estimating the properties of tissue-engineered cartilage.
组织工程软骨的结构和功能取决于生长过程中的组织培养条件和力学调节。可以使用诸如受限压缩试验等测试来评估组织工程软骨的质量。组织工程软骨构建体通常由外层软骨和内层核心组成，内层核心可以是未发育的软骨或正在降解的支架材料。使用双相多孔弹性有限元模型来证明这样的核心如何影响从受限压缩试验获得的反作用力-时间曲线。有限元模型预测，内层核心中更高体积的降解支架会降低根据受限压缩试验计算出的聚集模量，并提高组织渗透率的估计值。预测的聚集模量从均匀构建体的0.135MPa降低到仅70%为软骨的构建体的0.068MPa。研究发现，在估计组织工程软骨的特性时，应优先使用双相多孔弹性有限元建模，而不是假设均匀性的一维模型。
