霍乱患者血清中针对毒素协同调节菌毛抗体的检测。
Detection of antibodies to toxin-coregulated pili in sera from cholera patients.
作者信息
Attridge Stephen R, Wallerström Gun, Qadri Firdausi, Svennerholm Ann-Mari
机构信息
Department of Molecular Biosciences, The University of Adelaide, Adelaide, South Australia, Australia.
出版信息
Infect Immun. 2004 Mar;72(3):1824-7. doi: 10.1128/IAI.72.3.1824-1827.2004.
Abstract
Monoclonal antibodies (MAbs) were prepared against toxin-coregulated pili (TCP) isolated from Vibrio cholerae O1 El Tor. Despite their limited bactericidal potential, two MAbs were able to mediate biotype-specific protection against experimental cholera in infant mice. These MAbs were used in immunoblotting studies to assess seroconversion to El Tor TCP following cholera. Clear anti-pilus responses were observed in five of nine patients.
摘要
制备了针对从霍乱弧菌O1 El Tor分离的毒素共调节菌毛（TCP）的单克隆抗体（MAb）。尽管它们的杀菌潜力有限，但两种单克隆抗体能够介导对幼鼠实验性霍乱的生物型特异性保护。这些单克隆抗体用于免疫印迹研究，以评估霍乱后对El Tor TCP的血清转化。在9名患者中的5名中观察到明显的抗菌毛反应。
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