Tacket C O, Taylor R K, Losonsky G, Lim Y, Nataro J P, Kaper J B, Levine M M
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201, USA.
Infect Immun. 1998 Feb;66(2):692-5. doi: 10.1128/IAI.66.2.692-695.1998.
In this study, adult volunteers were fed tcpA and mshA deletion mutants of V. cholerae O139 strain CVD 112 to determine the role of toxin-coregulated pili (TCP) and mannose-sensitive hemagglutinin (MSHA) in intestinal colonization. Eight of 10 volunteers who received CVD 112 or CVD 112 delta mshA shed the vaccine strains in their stools; the geometric mean peak excretion for both groups was 1.4 x 10(5) CFU/g of stool. In contrast, only one of nine recipients of CVD 112 delta tcpA shed vibrios in his stool (P < 0.01); during the first 24 h after inoculation, 3 x 10(2) CFU/g was recovered from this volunteer. All recipients of CVD 112 and 8 (80%) of the recipients of CVD 112 delta mshA developed at least a fourfold rise in vibriocidal titer after immunization. In contrast, only one (11%) of the nine recipients of CVD 112 delta tcpA developed a fourfold rise in vibriocidal titer (P < 0.01). We conclude that TCP are an important colonization factor of V. cholerae O139 and probably of El Tor V. cholerae O1. In contrast, MSHA does not appear to promote intestinal colonization in humans.
在本研究中，成年志愿者被喂食霍乱弧菌O139菌株CVD 112的tcpA和mshA缺失突变体，以确定毒素共调节菌毛（TCP）和甘露糖敏感血凝素（MSHA）在肠道定植中的作用。接受CVD 112或CVD 112 ΔmshA的10名志愿者中有8人在粪便中排出了疫苗菌株；两组的几何平均峰值排泄量均为1.4×10⁵CFU/g粪便。相比之下，接受CVD 112 ΔtcpA的9名受试者中只有1人在粪便中排出了弧菌（P<0.01）；在接种后的头24小时内，从该志愿者身上回收了3×10²CFU/g。所有接受CVD 112的受试者以及8名（80%）接受CVD 112 ΔmshA的受试者在免疫后杀弧菌滴度至少升高了四倍。相比之下，接受CVD 112 ΔtcpA的9名受试者中只有1人（11%）的杀弧菌滴度升高了四倍（P<0.01）。我们得出结论，TCP是霍乱弧菌O139以及可能是埃尔托霍乱弧菌O1的重要定植因子。相比之下，MSHA似乎并未促进人类肠道定植。
