Liang Peiyu, Li Haoyong, Peng Xiaohui, Xiao Jinzhu, Liu Jihong, Ye Zhangqun
Department of Urology, First Affiliated Hospital, Hainan Medical College, Haikou, Hainan 570102, China.
Zhonghua Nan Ke Xue. 2004 Jan;10(1):42-5, 48.
To study the effects of astragalus membranaceus injection on sperm abnormality in Cd-induced rats.
Thirty adult male Sprague-Dawley rats were divided into 5 groups: low concentration abstragalus membranaceus group(A1), high concentration astragalus membranaceus group(A2), cyclophosphamide group (CP), cadmium chloride group (Cd) and control group (C). Astragalus membranaceus injections 5 g/(kg.d) or 10 g/(kg.d) were given intraperitoneally to the rats of the A1, A2, CP and Cd groups. After 7 days, cadmium chloride(0.2 mg Cd/kg body weight) was administrated by intraperitoneal injection stimultaneously to the A1, A2 and Cd group rats. At 22 d after cadmium chloride induction, the rats were sacrificed and the testis coefficient, testicular sperm count, daily sperm production, epididymal sperm count and percentage of sperm abnorality were observed, and the testes and epididymides were studied pathologically.
Testis coefficient, testicular sperm count, daily sperm production and epididymal sperm count in the A2 group [(5.68 +/- 1.19), (49.01 +/- 8.78) x 10(6)/g, (10.25 +/- 2.30) x 10(6)/(g.d), (47.51 +/- 22.51) x 10(6)/ml] increased significantly compared with those of the Cd group [(3.11 +/- 0.16), (37.59 +/- 10.63) x 10(6)/g, (5.31 +/- 0.32) x 10(6)/(g.d), (10.89 +/- 2.45) x 10(6)/ml] (P < 0.05 or P < 0.01). The percentage of sperm abnormality in the A2 group [(7.04 +/- 0.12)%] decreased significantly compared with that of the Cd group [(17.81 +/- 1.55)%] (P < 0.01).
As an antimutagen astragalus membranaceus has practical value in occupational protection against Cd-induced genetic damage.
研究黄芪注射液对镉诱导的大鼠精子异常的影响。
将30只成年雄性Sprague-Dawley大鼠分为5组：低浓度黄芪组（A1）、高浓度黄芪组（A2）、环磷酰胺组（CP）、氯化镉组（Cd）和对照组（C）。对A1、A2、CP和Cd组大鼠腹腔注射5 g/(kg·d)或10 g/(kg·d)的黄芪注射液。7天后，对A1、A2和Cd组大鼠同时腹腔注射氯化镉（0.2 mg Cd/kg体重）。在氯化镉诱导后22天，处死大鼠，观察睾丸系数、睾丸精子计数、每日精子生成量、附睾精子计数和精子异常率，并对睾丸和附睾进行病理研究。
A2组的睾丸系数、睾丸精子计数、每日精子生成量和附睾精子计数[分别为(5.68±1.19)、(49.01±8.78)×10⁶/g、(10.25±2.30)×10⁶/(g·d)、(47.51±22.51)×10⁶/ml]与Cd组[分别为(3.11±0.16)、(37.59±10.63)×10⁶/g、(5.31±0.32)×10⁶/(g·d)、(10.89±2.45)×10⁶/ml]相比显著升高（P<0.05或P<0.01）。A2组的精子异常率[(7.04±0.12)%]与Cd组[(17.81±1.55)%]相比显著降低（P<0.01）。
黄芪作为一种抗诱变剂，在职业性防护镉诱导的遗传损伤方面具有实际应用价值。
