Hidalgo Oriane, Garnatje Teresa, Susanna Alfonso, Mathez Joel
Botanical Institute of Barcelona (CSIC-Ajuntament de Barcelona), Av. Muntanyans, s.n., E-08038 Barcelona, Spain.
Ann Bot. 2004 Mar;93(3):283-93. doi: 10.1093/aob/mch042.
The monophyly of Valerianaceae and the precise delimitation of the family are not totally resolved. Our knowledge on the phylogeny of the group is only partial: on a morphological basis, some contradicting taxonomic proposals have been published, which demonstrates the difficulties in establishing a natural classification of the family and especially in proposing a relevant treatment of the large genus Valeriana. The aims of this study are to contribute to the phylogeny and generic delineation of the Valerianaceae on the basis of molecular data.
A cladistic analysis of the sequences of one plastid (matK) and one nuclear (ITS) molecular marker was carried out, both individually and in combination.
The results of the analyses of both regions confirm that the family is monophyletic, with the exclusion of Triplostegia. The tribe Patrinieae is monophyletic, and the tribe Valerianeae is also a natural group. Two of the subtribes of Valerianeae, Fediinae and Centranthinae, are also monophyletic, with the exclusion of the genus Plectritis from Fediinae. The subtribe Valerianinae, on the other hand, is paraphyletic.
Our results confirm, for the first time on a molecular basis, the suggested paraphyly of Valeriana in its present circumscription, with profound nomenclatural and taxonomic implications. The correlation between molecular phylogeny and biogeography is close. In the course of the plastid DNA sequencing, a polymorphism concerning the matK gene was found, a fact that should be carefully evaluated in phylogenetic analyses.
缬草科的单系性以及该科的精确界定尚未完全解决。我们对该类群系统发育的了解仅为部分：基于形态学，已发表了一些相互矛盾的分类学提议，这表明建立该科的自然分类存在困难，尤其是对大属缬草提出相关处理时。本研究的目的是基于分子数据为缬草科的系统发育和属的界定做出贡献。
对一个质体（matK）和一个核（ITS）分子标记的序列进行了分支分析，分别单独分析以及联合分析。
两个区域的分析结果均证实该科是单系的，但不包括双参属。败酱族是单系的，缬草族也是一个自然类群。缬草族的两个亚族，费迪亚亚族和矢车菊亚族，也是单系的，但费迪亚亚族不包括聚花草属。另一方面，缬草亚族是并系的。
我们的结果首次在分子基础上证实了当前界定的缬草属的并系性，具有深远的命名和分类学意义。分子系统发育与生物地理学之间的相关性密切。在质体DNA测序过程中，发现了与matK基因相关的多态性，这一事实在系统发育分析中应仔细评估。
