Sandra K, Devreese B, Van Beeumen J, Stals I, Claeyssens M
Laboratory of Protein Biochemistry and Protein Engineering, Ghent University, Ghent, Belgium.
J Am Soc Mass Spectrom. 2004 Mar;15(3):413-23. doi: 10.1016/j.jasms.2003.11.003.
The use of the recently introduced Q-Trap mass spectrometer in the study of protein glycosylation is described. The combined ion trap and triple quadrupole scan functions make it a powerful system in both oligosaccharide and glycopeptide analysis. Several oligosaccharides, both linear and branched, were analyzed to obtain information on sequence, linkage, and branching. Quadrupole like MS/MS spectra with ion trap sensitivity but without the typical ion trap low mass cut-off were obtained. To determine the origin of fragments and to reveal the existence of new ions, the MS(3) capabilities of the system proved to be useful. Glycopeptides were selectively detected in peptide mixtures using the triple quadrupole precursor ion scan function, either in off-line experiments or during LC/MS using information dependent acquisition (IDA).
本文描述了最近推出的Q-阱质谱仪在蛋白质糖基化研究中的应用。离子阱和三重四极杆扫描功能相结合，使其成为寡糖和糖肽分析的强大系统。分析了几种线性和分支寡糖，以获取有关序列、连接和分支的信息。获得了具有离子阱灵敏度但没有典型离子阱低质量截止的类似四极杆的串联质谱图。为了确定碎片的来源并揭示新离子的存在，该系统的MS(3)功能被证明是有用的。使用三重四极杆前体离子扫描功能，在离线实验中或在液相色谱/质谱联用过程中采用信息依赖采集（IDA），在肽混合物中选择性检测糖肽。
