Lee Yong-Keun, Song Jin, Lee Sang-Bae, Kim Kwang-Mahn, Choi Seong-Ho, Kim Chong-Kwan, LeGeros Racquel Z, Kim Kyoung-Nam
Medical Science and Engineering Research Center, Yonsei University College of Dentistry, Seoul 120-752, Korea.
J Biomed Mater Res A. 2004 Apr 1;69(1):188-95. doi: 10.1002/jbm.a.20137.
The purpose of this study was to investigate calcium phosphate glass as a potential biomaterial for hard tissue repair. We prepared calcium phosphate glass using the system CaO-CaF(2)-P(2)O(5)-MgO-ZnO and cultured MC3T3-E1 cells onto the glass in alpha-MEM with beta-glycerophosphatase and ascorbic acid. Proliferation of the cells was determined to evaluate the biocompatibility of the prepared calcium phosphate glass. The alkaline phosphatase activity was measured to examine the osteoblast differentiation. Mineralization was evaluated by staining the calcium precipitates with Alizarin red. Culture onto the calcium phosphate glass exhibited no significant influence on cell proliferation compared to the polystyrene chosen as a control in this experiment (p > 0.05). The alkaline phosphatase activity in the experimental group, however, was enhanced by the calcium phosphate glass significantly at 10-18 days after incubation than that of the control group (p < 0.05). The promotion of bone-like tissue formation by the calcium phosphate glass was observed after 7 days and thereafter. The results of the present study indicate that the prepared calcium phosphate glass affects osteogenesis by increasing calcification of the extracellular matrix.
本研究的目的是探究磷酸钙玻璃作为一种用于硬组织修复的潜在生物材料。我们使用CaO-CaF₂-P₂O₅-MgO-ZnO体系制备了磷酸钙玻璃，并将MC3T3-E1细胞接种到含有β-甘油磷酸酶和抗坏血酸的α-MEM培养基中的玻璃上。通过测定细胞增殖来评估所制备的磷酸钙玻璃的生物相容性。通过测量碱性磷酸酶活性来检测成骨细胞分化。用茜素红对钙沉淀进行染色来评估矿化情况。与本实验中作为对照的聚苯乙烯相比，接种到磷酸钙玻璃上对细胞增殖没有显著影响（p>0.05）。然而，实验组的碱性磷酸酶活性在培养10 - 18天后比对照组显著增强（p<0.05）。在7天及之后观察到磷酸钙玻璃促进了类骨组织的形成。本研究结果表明，所制备的磷酸钙玻璃通过增加细胞外基质的钙化来影响骨生成。
