The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
Biomed Mater. 2010 Jun;5(3):35004. doi: 10.1088/1748-6041/5/3/035004. Epub 2010 Apr 19.
This study aims to investigate the interaction between calcium silicate/calcium phosphate cement (CS/CPC) and osteogenesis, in particular the in vitro osteoblast-like and endothelial cells' response to CS/CPC. The effect of CS/CPC on cell attachment, proliferation and differentiation of murine osteoblast-like cell MC3T3-E1, as well as the influence on the cell attachment and proliferation of human umbilical vein endothelial cell (HUVEC), was studied in detail. Our results indicated that CS/CPC exhibited excellent biocompatibility to the osteoblast-like cells. Moreover, the morphology and cytoskeleton organization of MC3T3-E1 cultured on the CS/CPC disks suggested that CS/CPC induced better cell adhesion and cell spreading. Simultaneously, cell proliferation and alkaline phosphatase (ALP) activity of MC3T3-E1 were significantly improved after 3 and 7 days of culture on CS/CPC disks in comparison with CPC disks. Additionally, on CS/CPC disks, HUVEC attached well on day 1 and cell proliferation was also greatly enhanced by day 7. Collectively, these results suggest that the introduction of calcium silicate may improve the cell response involved in the osteogenesis and thus may be beneficial to further modify CPC as a better bone repairing material.
本研究旨在探究硅酸钙/磷酸钙水泥（CS/CPC）与成骨之间的相互作用，特别是 CS/CPC 对体外成骨细胞样和内皮细胞的反应。详细研究了 CS/CPC 对鼠源成骨细胞样细胞 MC3T3-E1 的细胞黏附、增殖和分化的影响，以及对人脐静脉内皮细胞（HUVEC）黏附增殖的影响。结果表明，CS/CPC 对成骨细胞样细胞具有优异的生物相容性。此外，CS/CPC 培养盘上 MC3T3-E1 的形态和细胞骨架组织表明，CS/CPC 诱导了更好的细胞黏附和细胞铺展。同时，与 CPC 培养盘相比，CS/CPC 培养盘上 MC3T3-E1 的细胞增殖和碱性磷酸酶（ALP）活性在培养 3 天和 7 天后显著提高。此外，在 CS/CPC 培养盘上，HUVEC 在第 1 天很好地黏附，第 7 天细胞增殖也大大增强。综上所述，这些结果表明引入硅酸钙可能会改善成骨过程中的细胞反应，从而可能有助于进一步改进 CPC 作为更好的骨修复材料。
