Tian Yuhui, Yang Xianjun, Li Wanli, Yang Rui, Yue Hanjun
Molecular Biology Laboratory, Xinxiang Medical College, Xinxiang 453003, China.
Wei Sheng Yan Jiu. 2003 Jul;32(4):361-2, 401.
To explore the effects of soybean isoflavone and calcium on proliferation, differentiation and mineralization of cultured osteoblastic cells. Osteoblastic cells from newborn rat calvarial was cultured. Osteoblastic cells proliferation was measured by MTT, activity of APL was observed with Golden's method and ARS was used to measure the mineral nodes of osteoblasts. The results showed that the calcium group had not different from the control group on stimulating the proliferation, differentiation and mineralization of rat calvarial osteoblastic cells. The soybean isoflavone group had significant effects on stimulating the proliferation, differentiation of osteoblasts and increase the forming of the mineral nodes of osteoblasts in rat. Calcium and soybean isoflavone group had more benefit for forming mineral nodes. The soybean isoflavone stimulates the proliferation, differentiation and mineralization of rat calvarial osteoblastic cells, and increase calcium in the same time is more benefit for mineralization.
探讨大豆异黄酮和钙对培养的成骨细胞增殖、分化及矿化的影响。培养新生大鼠颅骨来源的成骨细胞。采用MTT法检测成骨细胞增殖，用金氏法观察碱性磷酸酶（ALP）活性，并用茜素红染色法（ARS）检测成骨细胞的矿化结节。结果显示，钙组在刺激大鼠颅骨成骨细胞增殖、分化及矿化方面与对照组无差异。大豆异黄酮组对刺激大鼠成骨细胞增殖、分化及增加成骨细胞矿化结节形成有显著作用。钙与大豆异黄酮组对矿化结节形成更有益。大豆异黄酮刺激大鼠颅骨成骨细胞增殖、分化及矿化，同时补钙对矿化更有益。
