Cunningham F. X., Gantt E.
Department of Microbiology, University of Maryland, College Park, MD 20742; e-mail:
Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:557-583. doi: 10.1146/annurev.arplant.49.1.557.
Carotenoids are integral and essential components of the photosynthetic membranes in all plants. Within the past few years, genes encoding nearly all of the enzymes required for the biosynthesis of these indispensable pigments have been identified. This review focuses on recent findings as to the structure and function of these genes and the enzymes they encode. Three topics of current interest are also discussed: the source of isopentenyl pyrophosphate for carotenoid biosynthesis, the progress and possibilities of metabolic engineering of plants to alter carotenoid content and composition, and the compartmentation and association of the carotenogenic enzymes. A speculative schematic model of carotenogenic enzyme complexes is presented to help frame and provoke insightful questions leading to future experimentation.
类胡萝卜素是所有植物光合膜中不可或缺的重要组成部分。在过去几年里,编码这些不可或缺色素生物合成所需几乎所有酶的基因已被鉴定出来。本综述聚焦于这些基因及其所编码酶的结构与功能的最新研究发现。还讨论了当前三个备受关注的主题:类胡萝卜素生物合成中异戊烯基焦磷酸的来源、通过植物代谢工程改变类胡萝卜素含量和组成的进展与可能性,以及类胡萝卜素生成酶的区室化和缔合。本文提出了一个类胡萝卜素生成酶复合物的推测性示意图模型,以帮助构建并引发有见地的问题,从而推动未来的实验研究。