Park Si-Nae, Kim Jeoung Koo, Suh Hwal
Department of Biomedical Engineering, Yonsei University College of Medicine, Shinchon-dong, Seodaemun-ku, Seoul, South Korea.
Biomaterials. 2004 Aug;25(17):3689-98. doi: 10.1016/j.biomaterials.2003.10.072.
The 1-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride-crosslinked collagen-hyaluronic acid (HA) matrices containing tobramycin or ciprofloxacin as an antibiotic agent were fabricated for the control of wound contamination and characterized with respect to morphology, mechanical strength, in vitro release, antibacterial activity and cytotoxicity. For the tobramycin loaded matrix, the antibacterial capacity increased with the drug loading. Tobramycin and ciprofloxacin loaded matrices maintained their antibacterial effects for over 96 and 48 h, respectively. However, cell viability testing revealed that 0.4 mg/ml of ciprofloxacin has a cytotoxic effect on fetal human dermal fibroblasts. The effects of the bilayered collagen-HA matrices containing tobramycin and growth factors were also evaluated using an in vivo full thickness dermal defect model. Though the tobramycin incorporated collagen-HA matrix had no significant effect on wound healing compared with the control, the tobramycin incorporated matrix containing basic fibroblast growth factor or platelet-derived growth factor significantly enhanced wound healing. This study demonstrates the potential efficacy of crosslinked collagen-HA matrix containing antibiotics and growth factors for defective skin tissue replacement and infection prevention.
制备了含有妥布霉素或环丙沙星作为抗菌剂的1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐交联的胶原蛋白-透明质酸(HA)基质，用于控制伤口污染，并对其形态、机械强度、体外释放、抗菌活性和细胞毒性进行了表征。对于负载妥布霉素的基质，抗菌能力随药物负载量的增加而增强。负载妥布霉素和环丙沙星的基质分别在96小时和48小时以上保持其抗菌效果。然而，细胞活力测试显示，0.4mg/ml的环丙沙星对人胎儿真皮成纤维细胞具有细胞毒性作用。还使用体内全层皮肤缺损模型评估了含有妥布霉素和生长因子的双层胶原蛋白-HA基质的效果。尽管与对照相比，掺入妥布霉素的胶原蛋白-HA基质对伤口愈合没有显著影响，但掺入妥布霉素的含有碱性成纤维细胞生长因子或血小板衍生生长因子的基质显著促进了伤口愈合。本研究证明了含有抗生素和生长因子的交联胶原蛋白-HA基质在皮肤组织缺损修复和感染预防方面的潜在疗效。
