Matsuno Hiroaki, Yudoh Kazuo, Hashimoto Masamichi, Himeda Yasukazu, Miyoshi Teruzo, Yoshida Kaoru, Kano Syogo
The Center for Advanced Research of Biomedical Engineering, Toin University of Yokohama, 1614 Kurogane, Yokohama, 225-8502, Japan.
J Orthop Res. 2006 Mar;24(3):321-6. doi: 10.1002/jor.20070.
We have developed a novel bioabsorbable antibacterial carrier using hyaluronic acid (HA) gel for prevention and treatment of orthopedic infections. In this study, we investigated the in vivo antibacterial effects of two forms of this new material, an HA gel sponge and an HA gel film. A titanium cylinder was inserted into the intramedullary cavity of each rabbit femur, along with an HA gel sponge or HA gel film containing antibiotics. The HA gel sponge contained gentamycin, vancomycin, tobramycin, or minomycin. The HA gel film contained gentamycin or vancomycin. After 0, 7, and 14 days, the rabbit bone marrow was collected, and the antibacterial activity of the HA gel was determined by agar diffusion test. As a control, we used Septocoll, a commercially available antibacterial carrier. Both the HA gel sponge and HA gel film exhibited antibacterial activity. The present results indicate that HA gel containing antibiotics is a clinically useful bioabsorbable antibacterial carrier.
我们开发了一种新型的可生物吸收的抗菌载体，它使用透明质酸（HA）凝胶来预防和治疗骨科感染。在本研究中，我们研究了这种新材料的两种形式——HA凝胶海绵和HA凝胶膜在体内的抗菌效果。将一个钛圆柱体插入每只兔股骨的骨髓腔，同时放入含有抗生素的HA凝胶海绵或HA凝胶膜。HA凝胶海绵含有庆大霉素、万古霉素、妥布霉素或米诺环素。HA凝胶膜含有庆大霉素或万古霉素。在0、7和14天后，收集兔骨髓，并通过琼脂扩散试验测定HA凝胶的抗菌活性。作为对照，我们使用了Septocoll，一种市售的抗菌载体。HA凝胶海绵和HA凝胶膜均表现出抗菌活性。目前的结果表明，含抗生素的HA凝胶是一种临床上有用的可生物吸收的抗菌载体。
