Tinland B, Koukolíková-Nicola Z, Hall M N, Hohn B
Friedrich Miescher-Institut, Basel, Switzerland.
Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7442-6. doi: 10.1073/pnas.89.16.7442.
Agrobacterium tumefaciens causes neoplastic growth in plants by transferring a piece of DNA, called T-DNA, into the nucleus of the plant cell. The virulence protein VirD2 of A. tumefaciens is tightly linked to the T-DNA and is thought to direct it to the plant genome. Here we show that the VirD2 protein contains two nuclear localization signals that are functional both in yeast and in plant cells. One signal is located in the N-terminal part of the protein and resembles a single-cluster-type nuclear localization signal. The second signal is near the C terminus and is a bipartite-type nuclear localization signal. The involvement of these sequences in the entry of the T-DNA into the nucleus is discussed.
根癌土壤杆菌通过将一段称为T-DNA的DNA转移到植物细胞核中，从而导致植物发生肿瘤性生长。根癌土壤杆菌的毒力蛋白VirD2与T-DNA紧密相连，并被认为可将其引导至植物基因组。在此我们表明，VirD2蛋白包含两个在酵母和植物细胞中均具有功能的核定位信号。一个信号位于该蛋白的N端部分，类似于单簇型核定位信号。第二个信号靠近C端，是一种双分型核定位信号。本文讨论了这些序列在T-DNA进入细胞核过程中的作用。
