Yi Hana, Bae Kyung Sook, Chun Jongsik
School of Biological Sciences, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Korea.
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, Yusung, PO Box 115, Taejon 305-600, Korea.
Int J Syst Evol Microbiol. 2004 Mar;54(Pt 2):377-380. doi: 10.1099/ijs.0.02748-0.
A Gram-negative, aerobic, halophilic bacterium, designated strain JC2041(T), was isolated from a sediment sample of getbol, the Korean tidal flat. Results of 16S rDNA sequence analyses indicated a moderate relationship to Thalassomonas viridans within the gamma-Proteobacteria (94.9 % similarity). Depending on the tree-making algorithm used, the isolate either formed a monophyletic clade with T. viridans or was recovered as a sister group of a class containing the genera Thalassomonas and Colwellia. Phenotypic features of the getbol isolate were similar to those of T. viridans, but several physiological and chemotaxonomic properties, including nitrate reduction, amylase, lecithinase, Tweenase and utilization of 13 carbon sources, distinguished strain JC2041(T) from T. viridans. The polyphasic data presented in this study indicate that the isolate should be classified as a novel species in the genus Thalassomonas. The name Thalassomonas ganghwensis sp. nov. is therefore proposed for the getbol isolate; the type strain is JC2041(T) (=IMSNU 14005(T)=KCTC 12041(T)=DSM 15355(T)).
从韩国潮间带滩涂沉积物样本中分离出一株革兰氏阴性、需氧、嗜盐细菌，命名为菌株JC2041(T)。16S rDNA序列分析结果表明，该菌株与γ-变形菌纲中的绿色海单胞菌（Thalassomonas viridans）有一定亲缘关系（相似度为94.9%）。根据所使用的建树算法，该分离株要么与绿色海单胞菌形成一个单系分支，要么被归为包含海单胞菌属（Thalassomonas）和考氏菌属（Colwellia）的一个类群的姐妹群。潮间带分离株的表型特征与绿色海单胞菌相似，但包括硝酸盐还原、淀粉酶、卵磷脂酶、吐温酶以及13种碳源利用等在内的一些生理和化学分类学特性使JC2041(T)菌株与绿色海单胞菌有所区别。本研究中呈现的多相数据表明，该分离株应被归类为海单胞菌属中的一个新物种。因此，提议将该潮间带分离株命名为江华海单胞菌（Thalassomonas ganghwensis）新种；模式菌株为JC2041(T)（=IMSNU 14005(T)=KCTC 12041(T)=DSM 15355(T)）。
