Yi Hana, Bae Kyung Sook, Chun Jongsik
School of Biological Sciences, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, Yusung, PO Box 115, Taejon 305-600, Republic of Korea.
Int J Syst Evol Microbiol. 2004 Mar;54(Pt 2):571-576. doi: 10.1099/ijs.0.02798-0.
Two strictly aerobic, halophilic strains of the gamma-Proteobacteria, designated JC2042(T) and JC2043(T), were obtained from a sediment sample of getbol, the Korean tidal flat. Comparative 16S rDNA sequence studies revealed that the test strains were related most closely to the type strains of the genera Alteromonas (93.5-95.5 %) and Glaciecola (91.1-93.3 %). Phylogenetic analyses demonstrated that strains JC2042(T) and JC2043(T) formed a distinct monophyletic clade within the family Alteromonadaceae and clustered distantly with the genera Alteromonas and Glaciecola. Physiological, biochemical and chemotaxonomic data also indicated that the two getbol isolates were significantly different from members of these two genera and others with validly published names. Cells were rod-shaped and motile with a polar flagellum. The major isoprenoid quinone was Q8. The predominant cellular fatty acids were C(16 : 0), C(18 : 1)omega7c and a mixture of C(16 : 1)omega7c and iso-C(15 : 0) 2-OH. DNA G+C contents were 48-54 mol%. On the basis of this polyphasic study, Aestuariibacter gen. nov. is proposed with two novel species, Aestuariibacter salexigens sp. nov. (type strain, JC2042(T)=IMSNU 14006(T)=KCTC 12042(T)=DSM 15300(T)) and Aestuariibacter halophilus sp. nov. (type strain, JC2043(T)=IMSNU 14007(T)=KCTC 12043(T)=DSM 15266(T)). Aestuariibacter salexigens is the type species of the genus. In addition, an emended description of Alteromonas macleodii is proposed.
从韩国潮间带沉积物样本中分离出两株严格需氧、嗜盐的γ-变形菌，分别命名为JC2042(T)和JC2043(T)。16S rDNA序列比较研究表明，受试菌株与交替单胞菌属（93.5 - 95.5%）和嗜冷杆菌属（91.1 - 93.3%）的模式菌株关系最为密切。系统发育分析表明，菌株JC2042(T)和JC2043(T)在交替单胞菌科内形成一个独特的单系分支，与交替单胞菌属和嗜冷杆菌属的聚类关系较远。生理、生化和化学分类学数据也表明，这两株来自潮间带的分离株与这两个属以及其他已有效发表名称的菌属有显著差异。细胞呈杆状，具极生鞭毛，能运动。主要类异戊二烯醌为Q8。主要细胞脂肪酸为C(16 : 0)、C(18 : 1)ω7c以及C(16 : 1)ω7c和异-C(15 : 0) 2-OH的混合物。DNA G + C含量为48 - 54 mol%。基于此多相研究，提出了新属盐沼杆菌属，包含两个新种，即嗜盐盐沼杆菌新种（模式菌株，JC2042(T)=IMSNU 14006(T)=KCTC 12042(T)=DSM 15300(T)）和嗜盐盐沼杆菌新种（模式菌株，JC2043(T)=IMSNU 14007(T)=KCTC 12043(T)=DSM 15266(T)）。嗜盐盐沼杆菌是该属的模式种。此外，还对麦克劳德交替单胞菌提出了修订描述。
