Fardeau Marie-Laure, Salinas Monica Bonilla, L'Haridon Stéphane, Jeanthon Christian, Verhé Frédéric, Cayol Jean-Luc, Patel Bharat K C, Garcia Jean-Louis, Ollivier Bernard
IRD, UR101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
UMR CNRS 6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, 29680 Plouzané, France.
Int J Syst Evol Microbiol. 2004 Mar;54(Pt 2):467-474. doi: 10.1099/ijs.0.02711-0.
Novel thermophilic, anaerobic, Gram-positive, rod-shaped bacteria, strains SL9 and OCA1, were isolated from oilfields in France and Australia, respectively. Both strains, together with Thermoanaerobacter yonseiensis KB-1(T) (=DSM 13777(T)), Thermoanaerobacter tengcongensis MB4(T) (=DSM 15242(T)) and Carboxydibrachium pacificum JM(T) (=DSM 12653(T)), possessed genomic (DNA-DNA hybridization studies) and phylogenetic similarities with Thermoanaerobacter subterraneus SEBR 7858(T) (=DSM 13054(T)), which was isolated recently from an oilfield reservoir in south-west France. Marked phenotypic differences exist between the three oilfield isolates (T. subterraneus, strain OCA1 and strain SL9): they include temperature range for growth and substrates used. Differences were also observed in the DNA G+C contents of all organisms. Similarly to T. subterraneus, strains SL9 and OCA1, and also T. yonseiensis, T. tengcongensis and Carboxydibrachium pacificum, produced acetate and L-alanine as major end products of glucose metabolism [0.8-1.0 mol L-alanine produced (mol glucose consumed)(-1)] and reduced thiosulfate, but not sulfate, to sulfide. Because of these significant metabolic and phylogenetic differences between the oilfield isolates (T. subterraneus, strain OCA1 and strain SL9), T. yonseiensis, T. tengcongensis and Carboxydibrachium pacificum and other Thermoanaerobacter species, it is proposed to reassign them as a novel genus and species, Caldanaerobacter subterraneus gen. nov., sp. nov., comb. nov., with the creation of four novel subspecies, Caldanaerobacter subterraneus subsp. subterraneus subsp. nov., comb. nov., Caldanaerobacter subterraneus subsp. yonseiensis subsp. nov., comb. nov., Caldanaerobacter subterraneus subsp. tengcongensis subsp. nov., comb. nov. and Caldanaerobacter subterraneus subsp. pacificus subsp. nov., comb. nov.
新型嗜热厌氧革兰氏阳性杆菌菌株SL9和OCA1分别从法国和澳大利亚的油田中分离得到。这两个菌株与延世嗜热厌氧杆菌KB-1(T)（=DSM 13777(T)）、腾冲嗜热厌氧杆菌MB4(T)（=DSM 15242(T)）以及太平洋羧基双歧杆菌JM(T)（=DSM 12653(T)），在基因组（DNA-DNA杂交研究）和系统发育方面与最近从法国西南部油田储层中分离得到的地下嗜热厌氧杆菌SEBR 7858(T)（=DSM 13054(T)）具有相似性。这三株油田分离菌株（地下嗜热厌氧杆菌、OCA1菌株和SL9菌株）之间存在显著的表型差异：包括生长温度范围和所利用的底物。在所有生物体的DNA G+C含量方面也观察到差异。与地下嗜热厌氧杆菌类似，SL9和OCA1菌株，以及延世嗜热厌氧杆菌、腾冲嗜热厌氧杆菌和太平洋羧基双歧杆菌，产生乙酸盐和L-丙氨酸作为葡萄糖代谢的主要终产物[每消耗1摩尔葡萄糖产生0.8 - 1.0摩尔L-丙氨酸]，并将硫代硫酸盐而非硫酸盐还原为硫化物。由于这些油田分离菌株（地下嗜热厌氧杆菌、OCA1菌株和SL9菌株）、延世嗜热厌氧杆菌、腾冲嗜热厌氧杆菌和太平洋羧基双歧杆菌与其他嗜热厌氧杆菌属物种之间存在显著的代谢和系统发育差异，因此建议将它们重新分类为一个新的属和种，即地下卡尔厌氧杆菌属新属，新种，组合新名，同时创建四个新亚种，分别为地下卡尔厌氧杆菌地下亚种新亚种，组合新名、地下卡尔厌氧杆菌延世亚种新亚种，组合新名、地下卡尔厌氧杆菌腾冲亚种新亚种，组合新名和地下卡尔厌氧杆菌太平洋亚种新亚种，组合新名。
