Chades Theo, Scully Sean M, Ingvadottir Eva M, Orlygsson Johann
Faculty of Natural Resource Sciences, University of Akureyri, Akureyri, Iceland.
Front Microbiol. 2018 Aug 20;9:1931. doi: 10.3389/fmicb.2018.01931. eCollection 2018.
Mannitol-containing macro algae biomass, such as and , are a potential feedstock for the production of biofuels such as bioethanol. The purpose of this work was to evaluate the ability of thermophilic anaerobes within Class to ferment mannitol and mannitol-containing algal extracts. Screening of the type strains of six genera, , and ) was conducted on 20 mM mannitol and revealed that 11 of 41 strains could utilize mannitol with ethanol being the dominant end-product. Mannitol utilization seems to be most common within the genus of (7 of 16 strains) with yields up to 88% of the theoretical yield in the case of . Six selected mannitol-degrading strains (all species) were grown on mannitol extracts prepared from and . Five of the strains produced similar amounts of ethanol as compared with ethanol yields from mannitol only. Finally, was kinetically monitored using mannitol and mannitol extracts made from two macro algae species, and for end-product formation.
含甘露醇的大型藻类生物质,如[具体藻类1]和[具体藻类2],是生产生物乙醇等生物燃料的潜在原料。本研究的目的是评估[某类嗜热厌氧菌]中的嗜热厌氧菌发酵甘露醇和含甘露醇藻类提取物的能力。在20 mM甘露醇上对六个属[六个属的具体名称]的模式菌株进行筛选,结果显示41株中有11株能够利用甘露醇,乙醇是主要终产物。甘露醇利用似乎在[某属]中最为常见(16株中有7株),在[具体菌株]的情况下,产量高达理论产量的88%。选择了六株降解甘露醇的菌株(均为[具体物种]),在由[具体藻类1]和[具体藻类2]制备的甘露醇提取物上培养。与仅以甘露醇为原料的乙醇产量相比,其中五株菌株产生的乙醇量相似。最后,使用甘露醇和由两种大型藻类[具体藻类1]和[具体藻类2]制成的甘露醇提取物对[具体菌株]进行动力学监测,以观察终产物的形成。