Yoon Jung-Hoon, Yeo Soo-Hwan, Oh Tae-Kwang, Park Yong-Ha
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
The Centre for Traditional Micro-organism Resources, Keimyung University, Shindang-Dong, Dalseo-gu, Daegu, Korea.
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1197-1201. doi: 10.1099/ijs.0.63079-0.
A Gram-negative, motile, non-spore-forming, rod-shaped bacterium, designated strain TF-22T, was isolated from an intertidal sediment in Korea. This organism grew optimally at 30-37 degrees C and in the presence of 2-5% (w/v) NaCl. It did not grow without NaCl or in the presence of more than 14% (w/v) NaCl. Strain TF-22T was characterized chemotaxonomically as having ubiquinone-8 as the predominant respiratory lipoquinone and C(16 : 0), C(16 : 1) omega7c and/or iso-C(15 : 0) 2-OH and C(18 : 1) omega7c as the major fatty acids. The DNA G+C content of strain TF-22T was 46.0 mol%. Phylogenetic analyses based on 16S rDNA sequences showed that strain TF-22T falls within the gamma-subclass of the Proteobacteria and forms a coherent cluster with Alteromonas macleodii and Alteromonas marina. Levels of 16S rDNA similarity between strain TF-22T and the type strains of two Alteromonas species were in the range 98.1-98.6%. The level of DNA-DNA relatedness between strain TF-22T and the type strains of two Alteromonas species was 15.7-18.5%. Therefore, on the basis of phenotypic properties, phylogeny and genomic distinctiveness, strain TF-22T should be placed in the genus Alteromonas as a novel species, for which the name Alteromonas litorea sp. nov. is proposed. The type strain is TF-22TT (=KCCM 41775T=JCM 12188T).
从韩国潮间带沉积物中分离出一株革兰氏阴性、具运动性、不产芽孢的杆状细菌，命名为TF-22T菌株。该菌株在30-37℃及2-5%（w/v）NaCl存在的条件下生长最佳。在无NaCl或NaCl浓度超过14%（w/v）时不生长。TF-22T菌株经化学分类学鉴定，以泛醌-8作为主要呼吸性脂醌，主要脂肪酸为C(16 : 0)、C(16 : 1) ω7c和/或异-C(15 : 0) 2-OH以及C(18 : 1) ω7c。TF-22T菌株的DNA G+C含量为46.0 mol%。基于16S rDNA序列的系统发育分析表明，TF-22T菌株属于变形菌纲γ亚类，与麦克劳德交替单胞菌和海交替单胞菌形成一个连贯的聚类。TF-22T菌株与两种交替单胞菌模式菌株的16S rDNA相似性水平在98.1-98.6%之间。TF-22T菌株与两种交替单胞菌模式菌株的DNA-DNA相关性水平为15.7-18.5%。因此，基于表型特征、系统发育和基因组独特性，TF-22T菌株应作为一个新物种归入交替单胞菌属，为此提出新物种名为滨海交替单胞菌（Alteromonas litorea sp. nov.）。模式菌株为TF-22TT（=KCCM 41775T=JCM 12188T）。
