纤毛菌属的遗传多样性及新物种古德费洛纤毛菌、霍夫施塔德纤毛菌、沙氏纤毛菌和韦德纤毛菌的描述
Genetic diversity of Leptotrichia and description of Leptotrichia goodfellowii sp. nov., Leptotrichia hofstadii sp. nov., Leptotrichia shahii sp. nov. and Leptotrichia wadei sp. nov.
作者信息
Eribe Emenike R K, Paster Bruce J, Caugant Dominique A, Dewhirst Floyd E, Stromberg Verlyn K, Lacy George H, Olsen Ingar
机构信息
Institute of Oral Biology, Dental Faculty, University of Oslo, POB 1052, Blindern, N-0316 Oslo, Norway.
Department of Oral and Developmental Biology, Harvard School of Dental Medicine, Boston, MA 02115, USA.
出版信息
Int J Syst Evol Microbiol. 2004 Mar;54(Pt 2):583-592. doi: 10.1099/ijs.0.02819-0.
Sixty strains of Gram-negative, anaerobic, rod-shaped bacteria from human sources initially assigned to Leptotrichia buccalis (n=58) and 'Leptotrichia pseudobuccalis' (n=2) have been subjected to polyphasic taxonomy. Full-length 16S rDNA sequencing, DNA-DNA hybridization, RAPD, SDS-PAGE of whole-cell proteins, cellular fatty acid analysis and enzymic/biochemical tests supported the establishment of four novel Leptotrichia species from this collection, Leptotrichia goodfellowii sp. nov. (type strain LB 57(T)=CCUG 32286(T)=CIP 107915(T)), Leptotrichia hofstadii sp. nov. (type strain LB 23(T)=CCUG 47504(T)=CIP 107917(T)), Leptotrichia shahii sp. nov. (type strain LB 37(T)=CCUG 47503(T)=CIP 107916(T)) and Leptotrichia wadei sp. nov. (type strain LB 16(T)=CCUG 47505(T)=CIP 107918(T)). Light and electron microscopy showed that the four novel species were Gram-negative, non-spore-forming and non-motile rods. L. goodfellowii produced arginine dihydrolase, beta-galactosidase, N-acetyl-beta-glucosaminidase, arginine arylamidase, leucine arylamidase and histidine arylamidase. L. shahii produced alpha-arabinosidase. L. buccalis and L. goodfellowii fermented mannose and were beta-galactosidase-6-phosphate positive. L. goodfellowii, L. hofstadii and L. wadei were beta-haemolytic. L. buccalis fermented raffinose. With L. buccalis, L. goodfellowii showed 3.8-5.5 % DNA-DNA relatedness, L. shahii showed 24.5-34.1 % relatedness, L. hofstadii showed 27.3-36.3 % relatedness and L. wadei showed 24.1-35.9 % relatedness. 16S rDNA sequencing demonstrated that L. hofstadii, L. shahii, L. wadei and L. goodfellowii each formed individual clusters with 97, 96, 94 and 92 % similarity, respectively, to L. buccalis.
来自人类样本的60株革兰氏阴性厌氧杆状细菌，最初被鉴定为口腔纤毛菌（58株）和“假口腔纤毛菌”（2株），已进行了多相分类研究。全长16S rDNA测序、DNA-DNA杂交、随机扩增多态性DNA分析、全细胞蛋白的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、细胞脂肪酸分析以及酶学/生化试验支持从该菌库中确立4个新的纤毛菌物种，即古德费洛纤毛菌（Leptotrichia goodfellowii）新种（模式菌株LB 57(T)=CCUG 32286(T)=CIP 107915(T)）、霍夫斯塔德纤毛菌（Leptotrichia hofstadii）新种（模式菌株LB 23(T)=CCUG 47504(T)=CIP 107917(T)）、沙氏纤毛菌（Leptotrichia shahii）新种（模式菌株LB 37(T)=CCUG 47503(T)=CIP 107916(T)）和韦氏纤毛菌（Leptotrichia wadei）新种（模式菌株LB 16(T)=CCUG 47505(T)=CIP 107918(T)）。光学显微镜和电子显微镜观察表明，这4个新物种均为革兰氏阴性、无芽孢形成且不运动的杆菌。古德费洛纤毛菌可产生精氨酸双水解酶、β-半乳糖苷酶、N-乙酰-β-葡萄糖胺酶、精氨酸芳基酰胺酶、亮氨酸芳基酰胺酶和组氨酸芳基酰胺酶。沙氏纤毛菌可产生α-阿拉伯糖苷酶。口腔纤毛菌和古德费洛纤毛菌可发酵甘露糖，且β-半乳糖苷酶-6-磷酸呈阳性。古德费洛纤毛菌、霍夫斯塔德纤毛菌和韦氏纤毛菌具有β-溶血活性。口腔纤毛菌可发酵棉子糖。古德费洛纤毛菌与口腔纤毛菌的DNA-DNA相关性为3.8 - 5.5%，与沙氏纤毛菌的相关性为24.5 - 34.1%，与霍夫斯塔德纤毛菌的相关性为27.3 - 36.3%，与韦氏纤毛菌的相关性为24.1 - 35.9%。16S rDNA测序表明，霍夫斯塔德纤毛菌、沙氏纤毛菌、韦氏纤毛菌和古德费洛纤毛菌分别与口腔纤毛菌形成独立的聚类群，相似性分别为97%、96%、94%和92%。
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