Aricioglu Feyza, Ercil Eser, Dulger Gul
Department of Pharmacology, Faculty of Pharmacy, Marmara University, Istanbul, Turkey.
Ann N Y Acad Sci. 2003 Dec;1009:147-51. doi: 10.1196/annals.1304.016.
This study investigates the effects of agmatine on naloxone-precipitated withdrawal syndrome in morphine-dependent guinea pig ileum. Male guinea pigs that were starved for 24 hours were decapitated after cervical dislocation, and terminal portions of the ilea were removed. Segments were fixed at a resting tension of 1 g in an organ bath containing 1 x 10(-6) M morphine in Tyrode solution at 37 degrees C, which was bubbled with 95% O(2) and 5% CO(2). Tissues were incubated in morphine containing Tyrode solution for 4 hours before agmatine was added. Naloxone and agmatine had no effect on naive ilea. Naloxone (1 x 10(-6) M) contracted morphine-dependent ilea. Agmatine significantly inhibited the contractile response to naloxone in a dose-dependent manner (1 x 10(-7) M, 44%; 1 x 10(-6) M, 80%; 1 x 10(-5) M, 95%). This effect of agmatine was partly abolished by pretreatment with yohimbine and was almost completely abolished by idazoxan.
本研究调查了胍丁胺对吗啡依赖的豚鼠回肠中纳洛酮诱发的戒断综合征的影响。将禁食24小时的雄性豚鼠颈椎脱臼后断头，取出回肠末端部分。将肠段在含有1×10⁻⁶M吗啡的Tyrode溶液的器官浴中，于37℃以1g的静息张力固定，该溶液用95% O₂和5% CO₂鼓泡。在加入胍丁胺之前，将组织在含吗啡的Tyrode溶液中孵育4小时。纳洛酮和胍丁胺对未处理的回肠无影响。纳洛酮（1×10⁻⁶M）使吗啡依赖的回肠收缩。胍丁胺以剂量依赖方式（1×10⁻⁷M，44%；1×10⁻⁶M，80%；1×10⁻⁵M，95%）显著抑制对纳洛酮的收缩反应。胍丁胺的这种作用部分被育亨宾预处理消除，几乎完全被咪唑克生消除。
