Aricioglu Feyza, Utkan Tijen
Department of Pharmacology, Faculty of Pharmacy, Marmara University, Haydarpasa, Istanbul, Turkey.
Ann N Y Acad Sci. 2003 Dec;1009:185-9. doi: 10.1196/annals.1304.022.
Studies on the occurrence and properties of b-carbolines structurally related to harmala alkaloids have gained attention since it was hypothesized that some of these compounds play a role in processes of substance abuse and dependence. This study investigates the effects of harmane on naloxone-precipitated withdrawal syndrome in morphine-dependent guinea pig ileum. Segments of ilea from starved male guinea pigs were obtained and fixed at a resting tension of 1 g in an organ bath containing 10(-6) M morphine in Tyrode solution at 37 degrees C, which was bubbled with 95% O(2) and 5% CO(2). Tissues were incubated in 10(-6) M morphine containing Tyrode solution for 4 hours before harmane was added. Naloxone and harmane had no effect on naive ilea. Naloxone (10(-6) M) contracted morphine-dependent ilea. Harmane significantly inhibited the contractile response to naloxone in a dose-dependent manner (10(-7) M = 24%; 10(-6) M = 49.3%; 10(-5) = 70%). These results suggest that harmane may have beneficial effects on morphine withdrawal syndrome.
自从有人提出某些与哈尔满生物碱结构相关的β-咔啉化合物在药物滥用和成瘾过程中发挥作用以来，对其发生情况和性质的研究受到了关注。本研究调查了哈尔满对吗啡依赖的豚鼠回肠中纳洛酮诱发的戒断综合征的影响。取饥饿雄性豚鼠的回肠段，在37℃含10(-6)M吗啡的台氏液器官浴中以1g的静息张力固定，该溶液用95%O(2)和5%CO(2)鼓泡。在加入哈尔满之前，将组织在含10(-6)M吗啡的台氏液中孵育4小时。纳洛酮和哈尔满对未处理的回肠无影响。纳洛酮(10(-6)M)使吗啡依赖的回肠收缩。哈尔满以剂量依赖方式显著抑制对纳洛酮的收缩反应(10(-7)M = 24%；10(-6)M = 49.3%；10(-5)= 70%)。这些结果表明哈尔满可能对吗啡戒断综合征有有益作用。
