Velasco Leonardo, Mesa Socorro, Xu Chang-Ai, Delgado María J, Bedmar Eulogio J
Departamento de Biotecnología. Centro de Investigación y Desarrollo Agroalimentario, E-03150-La Alberca, Murcia, Spain.
Antonie Van Leeuwenhoek. 2004 Apr;85(3):229-35. doi: 10.1023/B:ANTO.0000020156.42470.db.
The nosRZDFYLX gene cluster for the respiratory nitrous oxide reductase from Bradyrhizobium japonicum strain USDA110 has been cloned and sequenced. Seven protein coding regions corresponding to nosR, nosZ, the structural gene, nosD, nosF, nosY, nosL, and nosX were detected. The deduced amino acid sequence exhibited a high degree of similarity to other nitrous oxide reductases from various sources. The NosZ protein included a signal peptide for protein export. Mutant strains carrying either a nosZ or a nosR mutation accumulated nitrous oxide when cultured microaerobically in the presence of nitrate. Maximal expression of a P nosZ-lacZ fusion in strain USDA110 required simultaneously both low level oxygen conditions and the presence of nitrate. Microaerobic activation of the fusion required FixLJ and FixK(2).
慢生根瘤菌USDA110菌株呼吸性一氧化二氮还原酶的nosRZDFYLX基因簇已被克隆并测序。检测到了与nosR、nosZ（结构基因）、nosD、nosF、nosY、nosL和nosX相对应的7个蛋白质编码区。推导的氨基酸序列与来自各种来源的其他一氧化二氮还原酶具有高度相似性。NosZ蛋白包含用于蛋白质输出的信号肽。携带nosZ或nosR突变的突变菌株在硝酸盐存在下进行微需氧培养时会积累一氧化二氮。USDA110菌株中P nosZ - lacZ融合体的最大表达同时需要低水平氧气条件和硝酸盐的存在。融合体的微需氧激活需要FixLJ和FixK(2)。
