Institute of Biochemistry, Faculty of Medicine, University of Giessen, Giessen, Germany.
Exp Parasitol. 2010 Dec;126(4):592-602. doi: 10.1016/j.exppara.2010.06.031. Epub 2010 Jul 1.
Comparative analyses of the N-glycosylation pattern of hemolymph glycoproteins from Biomphalaria glabrata strains Puerto Rico (BgPR) and Salvador (BgBS-90), differing in their susceptibility towards Schistosoma mansoni infection, were performed by Western blotting, enzyme-linked immunosorbent assays, two-dimensional high-performance liquid chromatography and mass spectrometry. Obtained data demonstrated an enhanced expression of serologically cross-reacting, fucosylated carbohydrate epitopes by the highly susceptible BgPR-strain in comparison to the resistant BgBS-90-strain. In particular, glycoproteins of BgPR snails exhibited larger amounts of glycans with (β1-2)-linked xylose or terminal Fuc(α1-3)GalNAc(β1-4)[±Fuc(α1-3)]GlcNAc(β1-)-units which are known to mediate cross-reactivity with schistosomal glycoconjugates. This finding could be corroborated by immunohistochemical studies showing again an enhanced expression of such carbohydrate epitopes in BgPR tissue. Hence, our results provide evidence for a correlation of B. glabrata susceptibility towards S. mansoni infection and the expression of carbohydrate determinants shared by the parasite and its intermediate host.
对来自易感性不同的两种品系（BgPR 和 BgBS-90）的血淋巴糖蛋白的 N-糖基化模式进行了比较分析，这两种品系分别是波多黎各品系（BgPR）和萨尔瓦多品系（BgBS-90），它们对曼氏血吸虫感染的易感性不同。通过 Western blot、酶联免疫吸附试验、二维高效液相色谱和质谱分析了这些品系。获得的数据表明，与抗性 BgBS-90 品系相比，高度易感的 BgPR 品系中具有血清学交叉反应的、岩藻糖基化碳水化合物表位的表达增强。特别是 BgPR 蜗牛的糖蛋白表现出更多具有（β1-2）连接的木糖或末端 Fuc（α1-3）GalNAc（β1-4）[±Fuc（α1-3）]GlcNAc（β1-）-单元的聚糖，已知这些聚糖与曼氏血吸虫糖缀合物具有交叉反应性。免疫组织化学研究再次证实了这种碳水化合物表位在 BgPR 组织中的表达增强，这一发现得到了证实。因此，我们的研究结果为 B. glabrata 对 S. mansoni 感染的易感性与寄生虫及其中间宿主共享的碳水化合物决定因素的表达之间存在相关性提供了证据。
