Unami Akira, Shinohara Yasuo, Kajimoto Kazuaki, Baba Yoshinobu
Faculty of Pharmaceutical Sciences, The University of Tokushima, Japan.
Biochem Pharmacol. 2004 Feb 1;67(3):555-64. doi: 10.1016/j.bcp.2003.09.010.
To characterize the energy metabolism in brown adipose tissue (BAT), the differences in gene expression profiles between BAT and white adipose tissue (WAT) were analyzed using a high-density cDNA microarray. RNAs isolated from two adipose tissues were hybridized to an Agilent rat cDNA Microarray that contained about 14,500 cDNA probe sets. The expression levels of 499 cDNA/ESTs were found to be at least 5-fold higher or lower in BAT than in WAT. Consistent with our previous findings, high expression levels of genes encoding uncoupling protein 1, muscle-type carnitine palmitoyltransferase and some other proteins involved in energy metabolism in BAT were found. Most of the genes encoding mitochondrial proteins, such as subunits of ATP synthase, cytochrome c oxidase, and NADH dehydrogenase, were highly expressed, reflecting possible differences in the cellular content of mitochondria between BAT and WAT. However, the expression levels of several genes encoding mitochondrial protein, such as liver mitochondrial aldehyde dehydrogenase and dicarboxylate carrier, were remarkably lower in BAT. These results may give important clues to understand the unique energy metabolism in BAT.
为了表征棕色脂肪组织（BAT）中的能量代谢，使用高密度cDNA微阵列分析了BAT与白色脂肪组织（WAT）之间基因表达谱的差异。从两种脂肪组织中分离的RNA与包含约14,500个cDNA探针组的安捷伦大鼠cDNA微阵列进行杂交。发现499个cDNA/EST的表达水平在BAT中比在WAT中至少高5倍或低5倍。与我们之前的研究结果一致，发现BAT中编码解偶联蛋白1、肌肉型肉碱棕榈酰转移酶和其他一些参与能量代谢的蛋白质的基因表达水平较高。大多数编码线粒体蛋白的基因，如ATP合酶亚基、细胞色素c氧化酶和NADH脱氢酶，都高度表达，这反映了BAT和WAT之间线粒体细胞含量可能存在差异。然而，BAT中一些编码线粒体蛋白的基因，如肝线粒体醛脱氢酶和二羧酸载体的表达水平明显较低。这些结果可能为理解BAT中独特的能量代谢提供重要线索。
