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Direct PCR of washed blood cells.

作者信息

Nordvåg B Y, Husby G, Raafat el-Gewely M

机构信息

Department of Rheumatology, Institute of Clinical Medicine, University Hospital of Tromsø, Norway.

出版信息

Biotechniques. 1992 Apr;12(4):490-3.

PMID:1503745
Abstract

We report a simple and rapid method for direct DNA amplification of washed blood cells by PCR. Small samples (2-100 microliters) of blood were washed, the cells resuspended in a buffer and used directly for PCR after boiling. Amplification of a specific DNA sequence of the human transthyretin gene, directed by the primers, was successfully performed. The method gives comparable results to amplifications made by purified DNA from blood.

摘要

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