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A simple method of DNA extraction from whole tissues and blood using glass powder for detection of transgenic animals by PCR.

作者信息

Attal J, Cajero-Juarez M, Houdebine L M

机构信息

Unité de Differentiation Cellulaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.

出版信息

Transgenic Res. 1995 Mar;4(2):149-50. doi: 10.1007/BF01969417.

DOI:10.1007/BF01969417
PMID:7704054
Abstract

A very simple and reliable method to extract DNA directly from mouse tail, rabbit ear and blood is described. Tissue was homogenized in a solution of NaI and the DNA was extracted using glass powder. The extracted DNA was obtained in sufficient quantity and purity to allow direct detection of transgene by PCR.

摘要

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Direct PCR of washed blood cells.
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