A simple method of DNA extraction from whole tissues and blood using glass powder for detection of transgenic animals by PCR.
作者信息
Attal J, Cajero-Juarez M, Houdebine L M
机构信息
Unité de Differentiation Cellulaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
出版信息
Transgenic Res. 1995 Mar;4(2):149-50. doi: 10.1007/BF01969417.
PMID:7704054
Abstract
A very simple and reliable method to extract DNA directly from mouse tail, rabbit ear and blood is described. Tissue was homogenized in a solution of NaI and the DNA was extracted using glass powder. The extracted DNA was obtained in sufficient quantity and purity to allow direct detection of transgene by PCR.
摘要
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