• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在大肠杆菌中表达的生物活性重组神经调节蛋白-1、-2和-3。

Bioactive recombinant neuregulin-1, -2, and -3 expressed in Escherichia coli.

作者信息

Mautino Beatrice, Dalla Costa Lorenza, Gambarotta Giovanna, Perroteau Isabelle, Fasolo Aldo, Dati Claudio

机构信息

Department of Animal and Human Biology, University of Torino, via Accademia Albertina 13, 10123 Torino, Italy.

出版信息

Protein Expr Purif. 2004 May;35(1):25-31. doi: 10.1016/j.pep.2003.12.012.

DOI:10.1016/j.pep.2003.12.012
PMID:15039062
Abstract

The neuregulins (NRGs) are a family of signaling proteins that are ligands for receptor tyrosine kinase of the ErbB family (namely ErbB3 and ErbB4). To date, four different neuregulin genes have been identified (neuregulin1-4). While NRG1 isoforms have been extensively studied, little is yet known about the other genes of the family. We report the expression of recombinant NRG1beta1, NRG2alpha, NRG2beta, and NRG3 as recombinant fusion proteins in Escherichia coli. The cDNA encoding for the EGF-like domain of each protein was cloned from the mouse olfactory bulb and inserted into the pET-19b vector allowing for bacterial expression of the protein fused to an N-terminal His tag. The recombinant NRGs expressed in the inclusion bodies were solubilized under denaturing conditions, purified by affinity chromatography, and refolded via dialysis in the presence of reducing agents. Purified recombinant NRGs were active as they bound to their receptors and induced their phosphorylation. In particular, and in agreement with data on the native proteins, all the molecules were able to bind and activate ErbB4 while only the rNRG1 and the two rNRG2 (but not rNRG3) bound ErbB3.

摘要

神经调节蛋白（NRGs）是一类信号蛋白，是ErbB家族受体酪氨酸激酶（即ErbB3和ErbB4）的配体。迄今为止，已鉴定出四种不同的神经调节蛋白基因（神经调节蛋白1 - 4）。虽然NRG1亚型已得到广泛研究，但该家族的其他基因仍知之甚少。我们报道了重组NRG1β1、NRG2α、NRG2β和NRG3作为重组融合蛋白在大肠杆菌中的表达。编码每种蛋白EGF样结构域的cDNA从小鼠嗅球中克隆出来，并插入到pET - 19b载体中，以实现与N端His标签融合的蛋白在细菌中的表达。在包涵体中表达的重组NRGs在变性条件下溶解，通过亲和层析纯化，并在还原剂存在的情况下通过透析复性。纯化后的重组NRGs具有活性，因为它们能与受体结合并诱导其磷酸化。特别是，与天然蛋白的数据一致，所有分子都能够结合并激活ErbB4，而只有rNRG1和两种rNRG2（而非rNRG3）能结合ErbB3。

相似文献

1
Bioactive recombinant neuregulin-1, -2, and -3 expressed in Escherichia coli.在大肠杆菌中表达的生物活性重组神经调节蛋白-1、-2和-3。
Protein Expr Purif. 2004 May;35(1):25-31. doi: 10.1016/j.pep.2003.12.012.
2
Neuregulin isoforms exhibit distinct patterns of ErbB family receptor activation.神经调节蛋白异构体表现出不同的ErbB家族受体激活模式。
Oncogene. 2002 Dec 5;21(55):8442-52. doi: 10.1038/sj.onc.1205960.
3
Purification and characterization of human and mouse recombinant alpha-fetoproteins expressed in Escherichia coli.在大肠杆菌中表达的人源和鼠源重组甲胎蛋白的纯化与特性分析
Protein Expr Purif. 1997 Jun;10(1):10-26. doi: 10.1006/prep.1996.0697.
4
Neuroglycan C, a novel member of the neuregulin family.神经聚糖C，神经调节蛋白家族的一个新成员。
Biochem Biophys Res Commun. 2004 Sep 3;321(4):1045-9. doi: 10.1016/j.bbrc.2004.07.066.
5
Amino-terminal charge affects the periplasmic accumulation of recombinant heregulin/EGF hybrids exported using the Escherichia coli alkaline phosphatase signal sequence.氨基末端电荷影响利用大肠杆菌碱性磷酸酶信号序列输出的重组人表皮生长因子受体（HER）配体/表皮生长因子（EGF）杂合体在周质中的积累。
Protein Expr Purif. 1997 Aug;10(3):331-9. doi: 10.1006/prep.1997.0741.
6
Characterization of a neural-specific splicing form of the human neuregulin 3 gene involved in oligodendrocyte survival.参与少突胶质细胞存活的人类神经调节蛋白3基因的神经特异性剪接形式的表征。
J Cell Sci. 2006 Mar 1;119(Pt 5):898-909. doi: 10.1242/jcs.02799. Epub 2006 Feb 14.
7
Neuregulin-3 (NRG3): a novel neural tissue-enriched protein that binds and activates ErbB4.神经调节蛋白-3（NRG3）：一种新的在神经组织中高表达的蛋白，可结合并激活ErbB4。
Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9562-7. doi: 10.1073/pnas.94.18.9562.
8
Cytotoxic activity of chimeric toxins containing the epidermal growth factor-like domain of heregulins fused to PE38KDEL, a truncated recombinant form of Pseudomonas exotoxin.
Cancer Res. 1995 Jan 1;55(1):71-7.
9
Neuregulin-2, a new ligand of ErbB3/ErbB4-receptor tyrosine kinases.神经调节蛋白-2，一种ErbB3/ErbB4受体酪氨酸激酶的新配体。
Nature. 1997 May 29;387(6632):512-6. doi: 10.1038/387512a0.
10
Recombinant human cytomegalovirus protease with a C-terminal (His)6 extension: purification, autocatalytic release of the mature enzyme, and biochemical characterization.具有C端（His）6延伸的重组人巨细胞病毒蛋白酶：纯化、成熟酶的自催化释放及生化特性分析
Protein Expr Purif. 1998 Dec;14(3):343-52. doi: 10.1006/prep.1998.0958.

引用本文的文献

1
Environmental Impacts of Cultured Meat: A Cradle-to-Gate Life Cycle Assessment.cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment.continue to next page cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle Assessment. cultured meat: A Cradle-to-Gate Life Cycle
ACS Food Sci Technol. 2024 Dec 30;5(1):61-74. doi: 10.1021/acsfoodscitech.4c00281. eCollection 2025 Jan 17.
2
Functional EGF domain of the human neuregulin 1α produced in Escherichia coli with accurate disulfide bonds.在大肠杆菌中产生的具有精确二硫键的人神经调节蛋白1α的功能性表皮生长因子结构域。
Mol Biol Rep. 2022 Dec;49(12):11715-11727. doi: 10.1007/s11033-022-07956-3. Epub 2022 Oct 5.
3
Extracellular Vesicles in Serum and Central Nervous System Tissues Contain microRNA Signatures in Sporadic Amyotrophic Lateral Sclerosis.血清和中枢神经系统组织中的细胞外囊泡在散发性肌萎缩侧索硬化症中含有微小RNA特征。
Front Mol Neurosci. 2021 Oct 29;14:739016. doi: 10.3389/fnmol.2021.739016. eCollection 2021.
4
Characterization of glial cell models and in vitro manipulation of the neuregulin1/ErbB system.神经调节蛋白1/表皮生长因子受体（ErbB）系统的神经胶质细胞模型表征及体外操作
Biomed Res Int. 2014;2014:310215. doi: 10.1155/2014/310215. Epub 2014 Aug 7.
5
Neuregulin1/ErbB4-induced migration in ST14A striatal progenitors: calcium-dependent mechanisms and modulation by NMDA receptor activation.神经调节蛋白 1/表皮生长因子受体 4 诱导纹状体祖细胞迁移：钙依赖性机制和 NMDA 受体激活的调节。
BMC Neurosci. 2011 Oct 12;12:103. doi: 10.1186/1471-2202-12-103.
6
Developmental regulation of metabotropic glutamate receptor 1 splice variants in olfactory bulb mitral cells.嗅球二尖瓣细胞中代谢型谷氨酸受体1剪接变体的发育调控
J Neurosci Res. 2009 Feb;87(2):369-79. doi: 10.1002/jnr.21864.
7
The role of NRG3 in mammary development.NRG3在乳腺发育中的作用。
J Mammary Gland Biol Neoplasia. 2008 Jun;13(2):195-203. doi: 10.1007/s10911-008-9082-8. Epub 2008 Apr 17.