[肺表面活性物质对豚鼠中耳积液的治疗作用]

[The curative effect of pulmonary surfactant on otitis media with effusion in guinea pigs].

作者信息

Huang Ping, Ma Zhaoxin, Chen Xuhui, Li Ming, Cao Yi, Cai Jianfeng

机构信息

Department of Otorhinolaryngology, Yueyang Hospital Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200437, China.

出版信息

Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2005 Jul;19(13):605-6, 610.

PMID:16200834

Abstract

OBJECTIVE

To find a new way of treating otitis media with effusion by studying the curative effect of pulmonary surfactant on secretory otitis media in guinea pigs.

METHOD

Nonviable heat-killed pneumococci (HKP) solution was inoculated into the middle ear cavity in guinea pig via a transeardrum approach, set up models of otitis media with effusion in guinea pigs, after being injected with pulmonary surfactant by transeardrum approach, observed changes of serous effusion in tympanum and response threshold.

RESULT

Five days after inoculation of HKB, serous effusion was present in the middle ear cavity of guinea pigs with disappearance of light cone. ABR response threshold raised from (14.00 +/- 3.08) dB to (45.00 +/- 5.67) dB. After seven days following injection of pulmonary surfactant by transeardrum approach, serous effusion of tympanum reduced or disappeared. ABR response threshold decreased from (45.00 +/- 5.67) dB to (23.53 +/- 6.32) dB, there was significantly difference between them (P < 0.001). In guinea pigs suffering from otitis media with effusion were not treated by PS, the serous effusion of tympanum did'nt change. Eardrum was more muddy and ABR response threshold raised from (45.00 +/- 5.67) dB before treatment (65.50 +/- 6.85) dB after being treated by PS, respectively, with statistically significant difference between them (P < 0.01).

CONCLUSION

Pulmonary surfactant plays an important role in treating otitis media with effusion in guinea pigs.

摘要

目的

通过研究肺表面活性物质对豚鼠分泌性中耳炎的疗效，寻找一种治疗中耳积液的新方法。

方法

采用经鼓膜途径将非活性热灭活肺炎球菌（HKP）溶液接种到豚鼠中耳腔，建立豚鼠中耳积液模型，经鼓膜途径注射肺表面活性物质后，观察鼓室浆液性渗出物的变化及反应阈值。

结果

接种HKB后5天，豚鼠中耳腔出现浆液性渗出物，光锥消失。听性脑干反应（ABR）反应阈值从（14.00±3.08）dB升高到（45.00±5.67）dB。经鼓膜途径注射肺表面活性物质7天后，鼓室浆液性渗出物减少或消失。ABR反应阈值从（45.00±5.67）dB降至（23.53±6.32）dB，两者之间有显著差异（P<0.001）。未用肺表面活性物质治疗的中耳积液豚鼠，鼓室浆液性渗出物无变化。鼓膜更浑浊，ABR反应阈值分别从治疗前的（45.00±5.67）dB升高到治疗后的（65.50±6.85）dB，两者之间有统计学显著差异（P<0.01）。