Gligic L, Vujovic N, Stevovic B, Manic J
Galenika A.D. Pharmaceutical Works, Institute for Research and Development, Department of Biotechnology, Batajnicki drum bb, Belgrado, Serbia.
Boll Chim Farm. 2003 Oct;142(8):330-2.
Growth and proliferation of brewer's yeast Saccharomyces cerevisiae in the presence of different Fe2+ levels was studied with the aim of finding optimal conditions for a maximum accumulation of bioavailable iron bound to constituents of yeast cells. The results demonstrated that iron stimulates growth and proliferation only under conditions of intensive aeration. Iron accumulation and the effect of aeration were examined in the presence 3.6 microM - 7.2 mM Fe2+, while its content in the cells after 20 h cultivation was determined by atomic absorption spectrophotometry. Control cultures were grown in no iron added medium. Further experiments revealed that iron concentrations ranging from 3.6 microM to 3.6 mM were beneficial to growth and proliferation, while higher levels did not affect these processes. The above range of iron concentrations also led to a more extensive iron accumulation, while further increase expressed no effect. So, Fe2+ concentration of 3.6 mM, enabling its high accumulation within the cells, while not negatively affecting biomass yield was selected for further studies. Iron uptake led to the modifications in transport of several other elements (Ca2+, Zn2+, K+ and Na+) and thus to the change in ion composition of the cells in comparison with the corresponding control.
为了找到使与酵母细胞成分结合的生物可利用铁最大程度积累的最佳条件，研究了酿酒酵母在不同Fe2+水平下的生长和增殖情况。结果表明，铁仅在强烈通气条件下刺激生长和增殖。在3.6 microM - 7.2 mM Fe2+存在的情况下检测了铁的积累和通气的影响，而培养20小时后细胞中铁的含量通过原子吸收分光光度法测定。对照培养物在不添加铁的培养基中生长。进一步的实验表明，3.6 microM至3.6 mM的铁浓度有利于生长和增殖，而更高的水平则不影响这些过程。上述铁浓度范围也导致更广泛的铁积累，而进一步增加则没有效果。因此，选择3.6 mM的Fe2+浓度，使其能够在细胞内大量积累，同时又不会对生物量产量产生负面影响，用于进一步研究。与相应的对照相比，铁的摄取导致其他几种元素（Ca2+、Zn2+、K+和Na+）的运输发生改变，从而导致细胞离子组成的变化。
