Hosokawa Keizo, Shibata Toshiro, Nakamura Ikuo, Hishida Atsuyuki
Department of Nutritional Management, Hyogo University, Kakogawa City, Hyogo, Japan.
Forensic Sci Int. 2004 Jan 28;139(2-3):195-9. doi: 10.1016/j.forsciint.2003.11.001.
An attempt was made to discriminate among six species of Papaver (P. bracteatum, P. orientale, P. pseudo-orientale, P. rhoeas, P. setigerum and P. somniferum) by comparing the nucleotide sequences of the plastid rpl16 gene and the rpl16-rpl14 spacer region. Comparison of sequences allowed us to distinguish five species, namely P. bracteatum, P. orientale, P. pseudo-orientale, P. rhoeas and P. setigerum plus P. somniferum from one another, but sequences from P. setigerum and P. somniferum were identical. It is difficult to distinguish between P. bracteatum, P. orientale and P. pseudo-orientale at the vegetative stage of growth. However, our method allowed us to distinguish between these three species and the others using nucleotide sequences and should allow identification of P. bracteatum that has been cultivated illegally in the garden in Japan. Furthermore, P. rhoeas was clearly discriminated from P. setigerum and P. somniferum by reference to the sequence of the rpl16 exon using young seedlings.
通过比较叶绿体rpl16基因和rpl16 - rpl14间隔区的核苷酸序列，尝试对罂粟属的六个物种（苞叶罂粟、东方罂粟、伪东方罂粟、虞美人、刚毛罂粟和罂粟）进行区分。序列比较使我们能够区分五个物种，即苞叶罂粟、东方罂粟、伪东方罂粟、虞美人、刚毛罂粟以及罂粟彼此之间的差异，但刚毛罂粟和罂粟的序列是相同的。在营养生长阶段，很难区分苞叶罂粟、东方罂粟和伪东方罂粟。然而，我们的方法使我们能够利用核苷酸序列区分这三个物种与其他物种，并且应该能够识别出在日本花园中非法种植的苞叶罂粟。此外，通过参考rpl16外显子的序列，利用幼苗可以清楚地将虞美人与刚毛罂粟和罂粟区分开来。
