Bakker Arjen H F, Van Dielen Francois M H, Greve Jan Willem M, Adam Judit A, Buurman Wim A
Department of Molecular Genetics, Toxicology Institute Maastricht (NUTRIM), University of Maastricht, Maastricht, The Netherlands.
Obes Res. 2004 Mar;12(3):488-98. doi: 10.1038/oby.2004.55.
To determine the variation in preadipocyte isolation procedure and to assess the number and function of preadipocytes from subcutaneous and omental adipose tissue of obese individuals.
The preadipocyte number per gram of adipose tissue in the abdominal-subcutaneous and abdominal-omental adipose stores of 27 obese subjects with a BMI of 44 +/- 10 kg/m(2) and an age of 40 +/- 9 years was determined.
The assessment of the preadipocyte number was found to be labor intensive and error prone. Our data indicated that the number of stromal vascular cells (SVCs), isolated from the adipose tissue by collagenase digestion, was dependent on the duration of collagenase treatment and the size and the origin of the biopsy. In addition, the fat accumulation and leptin production by differentiated SVCs were dependent on the number of adherent SVCs (aSVCs) in the culture plate and the presence of proteins derived from serum and peroxisome proliferator-activated receptor ligands.
Using our standardized isolation and differentiation protocol, we found that the number of SVCs, aSVCs, leptin production, and fat accumulation still varied considerably among individuals. Interestingly, within individuals, the number of SVCs, aSVCs, and the leptin production by differentiating aSVCs from both the subcutaneous and the omental fat depots were associated, whereas fat accumulation was not. In obese to severely obese subjects, differences in BMI and age could not explain differences in SVCs, aSVCs, leptin production, and fat accumulation.
确定前脂肪细胞分离程序的变化，并评估肥胖个体皮下和网膜脂肪组织中前脂肪细胞的数量和功能。
测定了27名肥胖受试者腹部皮下和腹部网膜脂肪库中每克脂肪组织的前脂肪细胞数量，这些受试者的体重指数（BMI）为44±10kg/m²，年龄为40±9岁。
发现前脂肪细胞数量的评估劳动强度大且容易出错。我们的数据表明，通过胶原酶消化从脂肪组织中分离出的基质血管细胞（SVCs）数量取决于胶原酶处理的持续时间、活检组织的大小和来源。此外，分化后的SVCs的脂肪积累和瘦素产生取决于培养板中贴壁SVCs（aSVCs）的数量以及血清衍生蛋白和过氧化物酶体增殖物激活受体配体的存在。
使用我们标准化的分离和分化方案，我们发现个体间SVCs、aSVCs的数量、瘦素产生和脂肪积累仍有很大差异。有趣的是，在个体内部，皮下和网膜脂肪库中SVCs、aSVCs的数量以及分化后的aSVCs产生的瘦素之间存在关联，而脂肪积累则不然。在肥胖至重度肥胖受试者中，BMI和年龄的差异无法解释SVCs、aSVCs、瘦素产生和脂肪积累的差异。
